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Effects of Insect-resistant Transgenic Cotton on Soil Microorganisms and Soil Enzyme Activity

Author: MaLiYing
Tutor: CuiJinJie
School: Chinese Academy of Agricultural Sciences
Course: Agricultural Entomology and Pest Control
Keywords: resist-transgenic cotton soil microorganisms diversity soil enzyme activity RAPD
CLC: S562
Type: Master's thesis
Year: 2009
Downloads: 35
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Abstract

In different periods of bearing, the quantity and various structure of soil microbiology, compounded with enzyme activity in soil, were evaluated in the laboratory in field and greenhouse.This paper is devoted to accumulating scientific evidences for secure evaluation of transgenic cotton and providing the evidences for potential danger on the production and soil ecosystem. The main research results are as follows:1. The effect of resist-transgenic cotton on the quantity of soil microorganisms By using the plate computation to do researches on the effects of the transgenic Bt cotton( han 5158,CCRI 30,GK12)and dual gene(Bt+CpTI) cotton(CCRI 41, sGK321) and the respective parental non-transgenic cotton under the two growing condition of field and greenhouse on the quantity of the main biome bacteria, fungi and actinomycetes.This paper clearly showed the changing trend of three kinds of cottons during the different periods of breeding. Under the condition of filed experiment, the changing trend of bacteria, fungi and actinomycetes was basically coherent.There was no difference in quantity of bacteria and actinomycetes between the two growing conditions.Under the two growing condition of field and greenhouse, the main biome was bacteria, and there was less actinomycetes. The quantity of fungi was the least among the transgenic cotton. The growing of han 5158 made the quantity of bacteria of different periods of breeding higher than non-transgenic cotton on the average. In the different periods of breeding,the quantity of fungi from the field soil planting the cotton CRRI 30,GK12 and sGK321, compareded with their control respectively,had increased respectively.2. The effect of resist-transgenic cotton on soil microorganisms’coloniaBased on the transgenic Bt cotton han 5158, dual gene (Bt+CpTI) cotton and respective parental non-transgenic cotton, the paper analyzed the effect on the structure of the soil microorganisms colonia.We selected twenty four RAPD random primers, among which, fifteen primers are capable of distinguishing the fingerprints.The results showed that there is no difference in the soil microorganism average enrichment, the Shannon-Weaver index of soil microorganisms and average degree in the four samples.3. The detecting of Bt gene in the soil of field planting resist-transgenic cottonBased on the transgenic Bt cotton( han 5158,CCRI 30,GK12),dual gene(Bt+CpTI) cotton(CCRI 41, sGK321) and the respective parental non-transgenic cotton, the paper detected the gene flow of transgenic Bt gene.We didn’t detect the fragment of Bt gene in the field which planted transgenic cotton many years.4. The effect of resist-transgenic cotton on the soil enzyme activity ?Based on the transgenic Bt cotton (han 5158,CCRI 30,GK12), dual gene (Bt+CpTI) cotton(CCRI 41,sGK321) and respective parental non-transgenic cotton, both on the field and greenhouse, the paper made an analysis on the effect on the the activities of soil urease,polyphenoloxidase, protease and phosphatase.The results showed that with the breeding process of cottons, the general changing trend was consistent, first rising and then falling, and until boll-opening rising again.The activity changing trend was different among polyphenoloxidase, protease, acid phosphatase and alkaline phosphatase.There were differences in the effect of different varieties during the different periods of breeding on the soil enzyme activity.Compareded with their parental non-transgenic cotton,the growing of transgenic Bt+CpTI cotton sGK321 under the conditions of field and greenhouse can decrease the activity of soil urease.Transgenic Bt cotton GK12 decreased the activity of polyphenoloxidase under the two conditions of field and greenhouse.

Effects of Insect-resistant Transgenic Cotton on Soil Microorganisms and Soil Enzyme Activity

Abstract

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