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Fluorescence Spectroscopy He-Ne laser interactions with blood

Author: BaiGuangFu
Tutor: HuLin
School: Guizhou University
Course: Theoretical Physics
Keywords: Fluorescence He-Ne laser Blood
CLC: R318.51
Type: Master's thesis
Year: 2009
Downloads: 13
Quote: 0
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Abstract

He-Ne laser irradiation therapy especially in clinical practice has been widely used, but its therapeutic mechanism has not been uniform understanding, on the one hand that the interaction between the laser and the complexity of the organism, on the other hand is that basic research is relatively less. This article was experimentally studied He-Ne laser irradiation on the fluorescence spectra of blood and effect change through spectral analysis of He-Ne laser on the role of biomass. This soil to innovation are: experimental observations before the He-Ne laser irradiation, the fluorescence spectra of blood components posterity; different power He-Ne laser irradiation the same time the fluorescence spectra; same power He-Ne laser irradiation of different time fluorescence spectra; through characteristic spectral analysis fluorescent substances in the blood changes in the structure; using non-harmonic oscillator model explains He-Ne laser irradiation of blood, the blood of the physical mechanism of fluorescence change, research in this area has not been reported . This article is divided into the following specific sections: The first chapter introduces the experimental use some basic theoretical knowledge: fluorescence spectra; laser-tissue interaction model; pair of He-Ne laser treatment done Mechanism Research Review; Finally topics in accordance with this article. The second chapter introduces the experimental principle and experimental methods, a brief introduction of the experimental apparatus and materials, meanwhile, described the experiment some important phenomena observed. Describes the sequence with the experimental results in order to carry out basically the same, first introduced the choice of excitation wavelength, thus introducing different circumstances He-Ne laser on whole blood, plasma, red blood cell fluorescence spectrum. The third chapter is mainly on the experimental results were analyzed and discussed. First, the related literature and reported in the experiment to determine the characteristic peak observed in the corresponding chromophore substances, and from the spectrum using wavelet analysis proposed in the blood of the characteristic peaks of porphyrins. Secondly, we used non-harmonic oscillator model explains He-Ne laser irradiation of blood induced changes in blood fluorescence spectra physical mechanism through analysis that He-Ne laser irradiation can stimulate or enhance blood molecular vibration, when the laser frequency with certain biomolecules close to the resonance frequency, resonance of biomolecules, leading to certain keys and branched chain was broken. For example, when a He-Ne laser irradiation of blood, the fiber protein and coenzyme branched or CC bond cleavage, conformational changes may be due to transitions caused by changes in the number, reflected in the fluorescence spectra is the peak fluorescence intensity change. Experimental results show that the He-Ne laser irradiation of blood in vitro, the use of laser irradiation 2mw 10min-20min, the fluorescence intensity before irradiation significantly enhanced compared. When increasing the laser power or the irradiation time, strength becomes weaker. The author believes that increasing the laser power or irradiation time inevitably leading to CC bond cleavage, at this time, the main quantum number has changed, vibrational energy level should be reduced. The fourth chapter is a summary of the full text of the work done, and to make some suggestions for future work and outlook.

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