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Objective:To observe the Cuscuta polysaccharide-hydroxyapatite complex partial implantation of the rabbit femoral condyle defect model of bone repair.Method:The 48 rabbits with bilateral femoral condyle to cancellous bone defect model, resulting in femoral condyle diameter 6mm, after the model of 10mm deep, were randomly divided into 3 groups (experimental group/control group/model group):The experimental group implants partial defect of Cuscuta polysaccharide-HA complex; control group, partial defect in control group implanted BMP-HA-gelatin complex; Model group of local non-implant defect area of drug.8,12 weeks after X ray imaging score after bilateral femoral condyle from bone samples of bone defects in general observation, histological examination and repair of bone defect area and the rate of bone defect repair statistics, observation of 3 groups the difference between.Result:Postoperative imaging score:8 weeks after imaging for the experimental group, score 3.071±0.475, control group,3.143±0.363, model group,1.167±0.389, the experimental group compared with the control group no significant difference, P> 0.05, compared with the model group P<0.001; 12-week experimental group was 3.571±0.514, control group,3.714±0.469, model group,1.833±0.835, the experimental group compared with the control group no significant difference P> 0.05, compared with the model group P<0.001.General observation:8 weeks, the experimental group, such as defects of soft tissue around the muscle slightly swollen, slightly white color, no congestion and edema, defect absorption implants can be seen clearly, and its soft texture obviously shows femoral condyle bone defect there is a large number of new bone callus growth to the Center from around the bone defect with new bone implants fuzzy boundaries; The control group, such as defects of soft tissue around the muscle with the experimental group cases, implants can be seen a small amount of absorption, but the experimental group than the high hardness and the edge of new bone defects less than the experimental group; Model group, such as defects of the muscles around the slight swelling of soft tissue, bone defect filling with red soft tissue, no significant bone growth.12 weeks, the experimental group, such as defects of soft tissue around the muscle swelling subsided, the color returned to normal, we can see a large number of defects generated callus and filling defects, color light than normal bone tissue, bone hardness of less than normal; The control group, such as defects of soft tissue around the muscle the situation with the experimental group, but bone less than the experimental group; Model group, such as defects of soft tissue around the muscle without swelling, without effusion, a small amount of surrounding bone to grow, it has hardened, we can see a larger soft tissue defect in the white area in which filling, and bone and soft tissue defect in the border area clear.Area of bone defect repair:8 weeks to repair bone defect area of the experimental group 21.558±0.933, control group,21.749±0.992, model group,17.003±1.423. Experimental group, the control group compared with the model group has a very significant difference (P<0.001) the experimental group, control group, no significant difference, P> 0.05; 12-week experimental group,25.654±0.662, control group,25.734±0.681, model group,20.543±0.798. Experimental group compared with the control group no significant difference (P> 0.05), compared with the model group P<0.001, a very significant difference.The rate of bone defect repair:8 weeks to repair bone defects in experimental group was 76.283±3.301, control group,76.961±3.509, model group,60.167±5.035. Experimental group compared with the control group no significant difference (P> 0.05), compared with the model group has a very significant difference (P<0.001); 12-week experimental group,90.777±2.341, control group,91.062±2.410, model group,72.694±2.822, Experimental group compared with the control group no significant difference (P> 0.05), compared with the model group has a very significant difference (P<0.001).Conclusion:Through 8 weeks,12 weeks, histological observation and imaging score, bone defect area, as well as the results of bone defect repair rate that Cuscuta polysaccharide-HA complex implantation femoral condyle of rabbit cancellous bone defect repair of a clear role its role with the BMP-HA-gelatin complex line.
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