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Objective To investigate the distribution of L-selectin gene P213S polymorphism in Han and Dongxiang nationality in Gansu province. To investigate the association with L-selectin gene P213S polymorphism and type 2 diabetes mellitus in Han and Dongxiang nationality.Methods Genotype of L-selectin was determined in 143 patients with type 2 DM and 152 healthy controls in Han nationality ; and 116 patients with type 2 DM and 126 healthy controls in Dongxiang nationality by polymerase chain reaction-ligation diction reaction(PCR-LDR) technique. Fasting plasma glucose, fasting insulin, blood fat, blood urea nitrogen, creatinine, uric acid were measured by biochemical technique. To measure height, weight, blood pressure, waistline, hip circumference, compute the body mass index (BMI), waist hip ratio (WHR) and Insulin Resistance Index (HOMA-IR).Results 1. There was no significant difference in L-selectin gene P213S polymorphism distributions in Han and Dongxiang nationality. 2. Genotype frequencies of PP, PS, SS were 55.9%, 37.8%, 6.3% in type 2 DM group and 41.4%, 50.7%, 7.9% in control group, allele frequencies of P, S were 74.8%, 25.2% in type 2 DM group and 66.8%, 33.2% in control group in Han nationality. There were significant differences in frequencies of genotype and allele between two groups(both P<0.05). 3. Genotype frequencies of PP, PS, SS were 44.8%, 48.3%, 6.9% in type 2 DM group and 38.1%, 44.4%, 17.5% in control group, allele frequencies of P, S were 69.0%, 31.0% in type 2 DM group and 60.3%, 39.7% in control group in Dongxiang nationality. There were significant differences in frequencies of genotype and allele between two groups(both P<0.05). 4. In type 2 DM group of Han nationality, the patients carrying PP, PS genotype had the significant increased levels of FPG with those who carrying SS genotype ( P<0.05). 5. In type 2 DM group of Dongxiang nationality, the patients carrying PP, PS genotype had the significant increased levels of FPG with those who carrying SS genotype ( P<0.05).Conclusion The data indicates that L-selectin gene P213S polymorphism may have some roles in the etiology of type 2 DM in Gansu Han and Dongxiang nationality.
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