Dissertation > Excellent graduate degree dissertation topics show

Sinomenine Inhibits Proliferation and Induces Apoptosis of Hela Cell Through PI3K/Akt Signaling Pathway

Author: TangSongZuo
Tutor: GaoYongXiang
School: Chengdu University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Sinomenine Hela cells Apoptosis Western blot COX-2 Akt Caspase-3
CLC: R285
Type: Master's thesis
Year: 2009
Downloads: 53
Quote: 0
Read: Download Dissertation

Abstract


Objective: To explore sinomenine in vitro induced apoptosis of human cervical cancer Hela cells , in order to found a Inhibition of cervical cancer cell growth by inducing apoptosis of tumor cells to new treatments ; explore Sinomenine apoptosis of cervical cancer Hela the molecular mechanisms of apoptosis , provide laboratory to explore the sinomenine the anti - cervical cancer drug . Methods: logarithmic phase Hela cells after treatment with sinomenine from morphological observation , MTT cell viability determination , flow cytometry analysis detected sinomenine on human cervical cancer Hela cells induced apoptotic effects . the immunoblot (WesternBlot) detection sinomenine treated human cervical cancer Hela cells , COX - 2 , p -Akt , Caspase -3 expression change . In Results: Sinomenine in vitro role of Hela cells , morphological observation the visible cell shrinkage , cytoplasmic condensation , refractive index enhancement , partially adherent cells round shrink to become suspended cells ; MTT assay of Hela vitality of cells , 0.625 mmol / L sinomenine 24 hours after Hela cell growth inhibition , 0.625mmol / L to 2.5mmol / L concentration range , with the increase in drug concentration , Green vine alkali Hela cell growth inhibition rate gradually increases ; flow cytometry analysis of apoptotic cells increased with the increase of the concentration of the drug . Western blot results suggest that the impact the sinomenine of COX-2 and apoptosis -related protein AKT and Caspase-3 expression , with increasing drug concentration , COX-2 and AKT decreased expression of Caspase -3 expression . Conclusion: sinomenine in vitro can significantly inhibit the proliferation of human cervical cancer Hela cells , this inhibition is achieved by Hela cells induced apoptosis , the mechanism may be by inhibiting the expression of COX-2 down Akt protein expression , increased caspase-3 expression work .

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. Based on statistics of the lognormal distribution heteroscedasticity model inferred,O212.1
  6. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  7. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  8. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  9. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  10. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  11. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  12. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  13. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  14. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  15. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  16. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  17. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  18. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  19. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  20. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  21. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile