Dissertation > Excellent graduate degree dissertation topics show

The Effect of Tα1 on the Antigen Presentation Ability of Dentritic Cells Derived from Children with Leukemia

Author: LiuXiaoDan
Tutor: LiXueRong;SunLiRong
School: Qingdao University
Course: Pediatrics
Keywords: dendritic cells thymosin alpha-1 tumor antigen acute leukemia Immune function children
CLC: R733.7
Type: Master's thesis
Year: 2011
Downloads: 17
Quote: 0
Read: Download Dissertation

Abstract


Objective To investigate the effect of thymosin alpha-1(Tα1) on the antigen presentation ability mediated by leukemia tumor antigen- loaded Dentritic Cells (DCs) derived from children acute leukemia(AL) peripheral blood in vitro. Method 10 acute lymphoblastic leukemia (ALL) children in complete remission were detected. The peripheral blood mononuclear cells (PBMC) samples of these children were obtained and divided into control group and test group respectively. The control group was cultured with rhGM-CSF+rhIL-4+rhTNF-a (GIT), while the test groups were cultured with rhGM-CSF+rhIL-4+rhTNF-a+HL-60 cells freeze thawing antigens (GITA、rhGM-CSF+rhIL-4+rhTNF-a+HL-60 cells freeze-thawing tumor antigen+ Tα1 (GITAT) respectively. The growth of DCs was observed under a optical microscope. We detected the immunephenotypes of DCs (CD1a、CD83、HLA-DR) by flow cytometry on the 8th day after cultured. We mixed the DCs from different groups with their autologous T cells, which were cultured with rhIL-2 for cytotoxic T cells (CTLs). We mixed the CTLs from different groups with HL-60 cells. Cytotoxic activities of different groups were measured by LDH assay.Results①All groups attained typical dentritic appearances on 8th day;②The difference of CDla+ cells between the test group and the control group was that the GITAT group was lower than the control group(P=0.028); and the rates of CD83+cells in the test group were both higher than the control group (P,=0.038, P2=0.010 respectively), the GITAT group was higher than the GITA group (P=0.045); the rates of HLA-DR+ cells in the test group were both higher than the control group (P1=0.020, P2=0.010 respectively), the GITAT group was higher than the GITA group (P=0.010)③The HL-60 as the target cells:the result of LDH assay showed that CTL killing activity in test group (GITA and GITAT) were higher than the control group(P=0.022, P=0.010), and killing activity of CTLs in the GITAT group was higher than the GITA group(P=0.034), the difference was significant。Conclusion①DCs loaded with freeze-thawed leukemia antigen are much riper which derive from children with leukemia in complete remission; DCs loaded with freeze-thawed HL-60 antigen induced CTL to develop more effective anti-leukemic immune effect in contrast wth the unloaded group. DCs loaded with freeze-thawed leukemia cells antigen also induced CTL to develop more effective anti-leukemic immune effect in contrast wth the unloaded group.②Thymosin alpha-1 can promoted the maturity of DCs and raised the cytotoxicity of CTL cooperating with rhGM-CSF, rhIL-4 and rhTNF-a. T a 1 can enhance the antigen-presentation ability of DC loaded with leukemia antigen derived from peripheral blood of children with acute leukemia.

Related Dissertations

  1. The Research of Children’s Medical Environment Based on New Medical Mode,TU246.1
  2. The Influence of Reputation on Preschool Children’s Transgression Behaviors,B844.1
  3. Social exclusion of migrant children in the Perspective of the New Exploration of compulsory education,G521
  4. Professor Kong Guangyi academic thought Qing Xuan main Treating children cold cough,clinical research and clinical experience,R249.2
  5. Professor Wang Yinglin academic thought and clinical experience and treatment of the palate and features of drug research in the original look of the children cold fever,R249.2
  6. Space environment of rural children left behind boarding schools,TU244.2
  7. Research on Present Situation and Influence Factors about Young Chrendren’s Tennis in Xi’an,G845
  8. The Flowing People’s Children Learning Adaptability and with Academic Self-efficacy and Mental Health Relationship,G441
  9. Study on Current Situation and Countermeasures to performative Children Basic Gymnastic in Shangdong Province,G613.7
  10. The Children of Migrant Workers Mental Health Counseling Strategy Research,G444
  11. Migrant Children’s Education Countermeasure Research,G521
  12. The Expression and Correlation Analysis of MT1A and MT1X in Patients with Acute Leukemia,R733.71
  13. Efficacy and Safety Evaluation of Claritin Treatment on Children with Asthma,R725.6
  14. Migrant workers' children equal right to education problem analysis,D412.6
  15. Children with acute leukemia also prototype folate carrier gene polymorphism,R733.71
  16. Research on Chinese Children's Songs 1950s and 1960s,J609.2
  17. Effects of Bovine Lactoferrin on the Immune Function and Intestinal Microflora of Chinese Mitten Crab (eriocheir Sinensis),S917.4
  18. Reference Values and Influence Factors of Fractional Exhaled Nitric Oxide for Healthy Children in Soochow,R446.1
  19. A Critical Discourse Analysis of Ideology in Kung Fu Panda,H313
  20. The Research on Street Children’s Relief in the View of the Social Capital Theory,D632.1
  21. The Application of Art Therapy to the Art Education for Children,G623.75

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile