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The Effect of Vitamin E and Selenium on Apoptosis and Oxidative Damage of Human Thyrocyte Induced by H2O2

Author: ShiShaSha
Tutor: YanShengLi
School: Qingdao University
Course: Endocrine and metabolic diseases
Keywords: vitamin E thyroid selenium apoptosis oxidative damage
CLC: R581
Type: Master's thesis
Year: 2011
Downloads: 40
Quote: 0
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Abstract


Objective To investigate the influence of vitamin E(VE) and selenium(Se) on apoptosis and oxidative damage of human thyroid epithelium cells(TEC) induced by H2O2.Methods The human thyroid epithelium cells from normal para-adenoma tissues of patients with thyroid adenoma resection were cultured. In prevention groups, vitamin E(50μmol/L), Selenium(10-7mol/L)or serum-free medium was respectively added into thyrocytes of monolayer culture before H2O2 (200～800μmol/L) with different concentrations added, while in treatment groups H2O2 (200-800μmol/L) was added firstly. The survival rates of thyrocyte were detected by MTT stain, and the apoptosis rates by flow cytometry. The activity of GSH-Px and content of MDA were determined.Results When the thyroid epithelium cells exposed to H2O2 (200-800μmol/L)only for 24 hours, the cell survival rates declined(P<0.01),cell apoptosis rates increased (P<0.01), the GSH-Px activity decreased (P<0.05)and MDA content increased(P<0.01) in both prevention and treatment groups, with a relation of dose-effect response. Adding VE(50μmol/L)and Se(10"7mol/L)into cell culture for prevention and treatment, the cell survival rates increased, the thyrocyte apoptosis reduced, the activity of GSH-Px increased and the content of MDA decreased compared to the corresponding simple H2O2 intervention groups. Above differences were particularly significant(P<0.05) when thyrocytes exposed to 400 and 800μmol/L H2O2. The decrease of cell apoptosis rates in group with selenium pre-intervention was more significant than that in group with VE pre-intervention(P=0.018) when cells exposed to 400μmol/L H2O2.Besides, compared with that in group with VE, the reduction of MDA content in group with selenium was more obvious (P<0.05) when cells exposed to 200 and 800μmol/L H2O2 in prevention groups. While in treatment groups,the decline of cell apoptosis rates in group with VE was more obvious than that in group with selenium (P<0.05) when cells exposed to 200 and 400μmol/L H2O2 and the increase of GSH-Px activity in group with selenium was more significant than that in group with VE(P=0.014) when cells exposed to 400μmol/L H2O2. Conclusion Vitamin E and selenium could have protective effect on apoptosis and oxidative damage induced by H2O2 in human thyrocytes.

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CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Thyroid disease
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