Dissertation > Excellent graduate degree dissertation topics show

Expression of RRM1 and MDR1 in Cervical Squamous Cell Carcinoma and Its Relationship with the Chemo-sensitivity to NACT

Author: RenZuoZuo
Tutor: WangLiMing
School: Qingdao University
Course: Obstetrics and Gynaecology
Keywords: Cervical cancer RRM1 MDR1 NACT Drug resistance
CLC: R737.33
Type: Master's thesis
Year: 2011
Downloads: 9
Quote: 0
Read: Download Dissertation


Objective To study the expression of Ribonucleotide Reductase M1 (RRM1) and Multi-drug Resistance 1(MDR1) in cervical squamous cell carcinoma, the relationship of RRM1, MDRl and the relationship with the chemo-sensitivity to NACT.Method Using the immunohistochemistry PV-6000 and RT-PCR, we comparatively studied the expression of RRM1 and MDR1 on 40 cases of cervical squamous cell who accepted NACT. To analyze the expression of RRM1 and MDR1 in cervical squamous cell carcinoma, the relationship of RRM1,MDR1 and the relationship with the chemo-sensitivity to NACT through Pearson chi-square test, Fisher’s exact test, t test and Spearman correlations.Result 1、The result of immunohistochemistry showed:After NACT, the expression of RRMl in drug resistance group was not higher than before (x2=2.763, P=0.096). And the expression of RRM1 in drug sensitive group was no significant change (x2=3.247, P=0.072). The result of RT-PCR showed: after NACT, the expression of RRM1 in drug resistance group was higher than before (t=2.284, P=0.037), but in drug sensitive group was no significant change (t=0.861, P=0.398)2、The expression of RRM1 was correlated with the expression of MDR1 [Before NACT:(the result of immunohistochemistry showed:r=0.802, P=0.000; the result of RT-PCR showed:r=0.377, P=0.016). After NACT:(the result of immunohistochemistry showed:r=0.771, P=0.000; the result of RT-PCR showed:r=0.407, P=0.010)].3、Before NACT, RRM1 negative group of chemotherapy response rate (75.0%) was higher than RRM1 positive effective rate (37.5%) (x2=5.625, P=0.018); the result of RT-PCR showed the expression of RRM1 in drug resistance group was higher than in drug sensitive group (t=2.086.P=0.044)Conclusion RRM1 and MDR1 expressed in cervical cancer together. NACT may raise the level of RRM1 expression, and both of them possibly participated in the process of platinum resistance. So RRM1 should be further investigated as the predictive markers for the chemo-sensitivity to NACT in cervical cancer.

Related Dissertations

  1. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  2. Clinical Research of Bacteria Spectrum and the Change of Bacterial Drug Resistance About Pediatric Diseases During Six Years,R446.5
  3. Effect of Proteasome Inhibitor Bortezomib on MDR1 Gene Expression in K562 Cell,R733.7
  4. Expression Characteristics and Clinical Significance of RECK in Cervical Cancer,R737.33
  5. Cervical Cancer Patients Clinical Prognostic Factor Analysis (72 Cases Analysis Attached),R737.33
  6. Effect of the Function of MRP1 on Human Lung Adenocarcinoma Chemotherapy Resistance,R734.2
  7. Association of Expression of ERCC1/RRM1 with Survival and Resistance to Platinum Inpatients with Ⅰ-ⅢA NSCLC,R734.2
  8. MDR1 and CYP3A gene polymorphisms on digoxin plasma concentrations of,R96
  9. Diffusion -weighted imaging and dynamic contrast-enhanced scan in cervical cancer staging and therapeutic monitoring applications,R737.33
  10. Lesion Bacterial Investigation of Patients Suffer from Cutaneous Squamous Cell Carcinoma,R739.5
  11. Reversing Effect of Curcumin on Multi-drug Resistance of Human Hepatocellular Carcinoma Bel7402/5-Fu Cell Line,R285.5
  12. Effect of STAT1-shRNA-mediated Gene Silencing on Biological Characteristics in the Cervical Cancer Cell Line Hela,R737.33
  13. Expression of COX-2 and Hif-1α, and Its Correlation with Microvascular Angiogenesis in Cervical Cancer,R737.33
  14. Detection and Significance of Dendritic Cell in Xinjiang Uygur Women Cervical Intraepithelial Neoplasia and Cervical Cancer,R737.33
  15. Correlation of MDR1, P53 and VEGF Expression and Prognosis in Gastric Carcinoma,R735.2
  16. Association of the MDR1 3435 Polymorphism in Patients with Refractory Rheumatoid Arthritis in a Chinese Han Population,R593.22
  17. The Relationship Between XRCC1 Polymorphisms and the Risk of Cervical Cancer in Jiangsu Population,R737.33
  18. The Study on the Expression of hWAPL Gene in CIN and Cervical Squamous Carcinoma,R737.33
  19. Correlation between Expression of Maspin and Bax with Efficacy of Neoadjuvant Chemotherapy for Cervical Carcinoma,R737.33
  20. Associoation of MDR1 Gene Methylation Status with NACT Effects of Cervical Cancer,R737.33

CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
© 2012 www.DissertationTopic.Net  Mobile