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Dai Lake,the area of 30 Mu,lies in the dingle between Jinyun Mountain and the North Hot Spring with a richful resources of freshwater microalgae.So it is called as the "Chongqing freshwater microalgae gene pool".The majority species are belongs to the Bacillariophyta and Chlorophyta. Microalgae has a lot of traits such as small,nutrient,rapid growth and reproduction,rich in fats, strong viability to the environment,easy to culture and so on.So people pay a high attention on it. Microalgae has been widely used in food industrial,Agriculture and industry.Therefore,the research and in vitro Culture of microalgae resource are a significance matter.Because of the richful fats,microalgae is considered as an good raw materials to pruduce the biodiesel oil for the important substitute of the fossil energy.The main freshwater microalgar with potential development are Bacillariophyta and Chlorophyta.Although there is a plenty of Bacillariophyta and Chlorophyta resources in the Dai Lake of Jinyun Mountain,energymicroalgae have not been researched in this lake so far.So this experiment trys to use in vitro Culture of Bacillariophyta and Chlorophyta.The intention of this experiment is to probe into some good in vitro culture system for Bacillariophyta and Chlorophyta under the lab condition and to primarily identified the Bacillariophyta and Chlorophyta in the systems of in vitro Culture those will provide a scientific data for rational utilization of microalgae resources in Dai lake.Using knop medium in this experiment,the water to isolate and culture microalgae from Dai Lake. Then we use f/2 and the 4th medium to isolate and culture Diatom and Chlorella.Finally we identifiy species of Diatom and Chlorella that we cultured by morphology characterThe experimental results are summarized as follows:1 Established optimization culture system of Bacillariophyta:f/2 medium,time rate of day/ night12:12,the temperature 26.5℃,relative humidity 85%～100%,pH 6.8,light intensity 3000～4000Lx.2 Established optimization culture system of Chlorophyta:the HB 4 medium,time rate of day /night 12:12,the temperature 26.5℃,relative humidity 85%～100%,pH 6.8,light intensity 3000～4000Lx.3 The 36 species of Bacillariophyta seperated and cultured on f/2 medium were identified,which were belonges to the 2 classes,4 orders,6 families and 5 genuses.4 The 35 species of primary Chlorophyta seperated and cultured on the Shuisheng 4# medium were identified,which were belonges to the to the 2 classes,3 orders,9 families and 18 genuses. The enery microalgae seperated in the experiments are:Cyclotella meneghiniana Kütz., Navicula bacillum Ehr.,Navicula rhynchocephala Kütz.,N.dicephala(Ehr.)W.Smith,N. graciloides May.,N.radiosa Kütz.,Scenedesmus dimorphus(Turp.)Kütz and S.quadricauda (Turp.)Bréb.
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