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Preparation of Enzyme Carrier by Biosilification and Self-assembly for CO2 Conversion

Author: SunZuoZuo
Tutor: JiangZhongYi
School: Tianjin University
Course: Chemical processes
Keywords: biomimetic silicification self-assembly silica protamine enzyme carrier encapsulation
CLC: Q814.2
Type: Master's thesis
Year: 2009
Downloads: 41
Quote: 0
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Abstract


The astonishing ability of living organisms to produce biocomposites with superior characteristics attracts the growing attention of numerous research groups. Biomimetic mineralization, which utilizes biological molecules or synthetic analogues to template and catalyzes the formation of inorganic oxides under mild conditions, provides a versatile new technology for enzyme immobilization with several inherent advantages. The morphology of the inorganic materials can be controlled by the template self-assembly. The process of LbL self-assembly is conducted in aqueous solution under mild conditions, which can well preserve the enzymes native structures and activities. Both of the biomimetic mineralization and self-assembly process can create benign microenvironment for the immobilized enzyme. This study discusses biomimetic and self-assembly approach for the formation of silica-based materials and their potential application in the preparation of enzyme carriers.The details in this study were summarized as follows:Firstly, mimicking the nacre layer in mollusk shell, a novel approach combining biomimetic mineralization with layer-by-layer (LbL) self-assembly was proposed to prepare protamine–silica hybrid microcapsules. More specifically, these microcapsules were fabricated by alternative deposition of positively charged protamine layers and negatively charged silica layers on the surface of CaCO3 microparticles, followed by dissolution of the CaCO3 microparticles using EDTA. Moreover, these protamine-silica hybrid microcapsules were employed as the carrier for the immobilization of YADH. The encapsulated YADH displayed enhanced recycling and storage stability.Secondly,inspired by the structure of cell, a microfactory with capsules-in- capsule structure was prepared for multienzymes immobilization. The Alg-Pro-Si capsules were loaded with LbL self-assembly microcapsules where the Alg-Pro-Si capsules worked as structural shell and the LbL microcapsules worked as“workshop”. By encapsulating different enzyme-containing LbL microcapsules within Alg-Pro-Si capsules, an enzymatic‘assembly line’could be created to alter the incoming molecules or‘manufacture’the product. The reaction probabilities and efficiencies would be considerably enhanced owing to spatial confinement of the biochemical machinery. The enzymes encapsulated by Capsules-in-capsule micro reactor displayed higher methanol yield and stability than free enzymes system.

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CLC: > Biological Sciences > Bioengineering ( Biotechnology ) > Enzyme Engineering > Immobilized enzyme and immobilized cell technology
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