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Relationship between Biodiversity of Deuteromycetes in Sediment and Marine Contamination Along the Coast Line

Author: ShiQuanYi
Tutor: TianLi
School: Qingdao University of Science and Technology
Course: Biochemical Engineering
Keywords: marine contamination Deuteromycetes population biodiversity
CLC: X55
Type: Master's thesis
Year: 2009
Downloads: 15
Quote: 0
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Abstract


The contents of N, P, heavy metal in Kiaochow bay coast of Licun river and Haibo river estuaries sediments collected in winter, spring and autumn were measured with Shilaoren as control. Statistical analysis of contamination status and biodiversity of Deuteromycetes in these habitats were conducted using traditional methods combined with modern 18S PCR-DGGE methods. The results showed Licun river estuaries was seriously polluted while Haibo river estuaries was moderate polluted and the amount of culturable Deuteromycetes decreased as the increase of pollution degree and this tendency was obviously relevant with heavy metal pollution degree. However, the tendency of Deuteromycetes biodiversity was different: within slight to moderate pollution scope, the biodiversity index of Deuteromycetes increased as the increase of heavy metal index while the biodiversity index of Deuteromycetes decreased sharply within seriously pollution scope. The change of total N, P could only exert effect on some Deuteromycetes populations and had little effect on the amount and biodiversity of the integrity Deuteromycetes. The innovation point of this study lies in the fact that deuteromycete was chosen as marine environmental microorganism indicator to investigate a variety of polluted sites including Licun River estuary region (heavy polluted area), Haibo River estuary region(medium polluted area) with the Shilaoren beach bathe (clean area) as control. Molecular methods such as 18S-PCR denaturing gradient gel electrophoresis (18S PCR-DGGE) combined with traditional culture method were utilized to analysis the relevance between Deuteromycete population diversity and heavy metal pollution in three seasonsand the resultes strongly suggested that Deuteromycete could be used as a reliable marine pollution microorganism indicator. A relevance model between Deuteromycete population, Deuteromycete diversity and marine pollution was primarily designed on the basis of above obtained results and established foundation for further marine pollution monitoring.

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CLC: > Environmental science, safety science > Environmental pollution and its prevention > Marine pollution and its prevention
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