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Improving on the Ethanol Yield of Saccharomyces Cerevisiae Through Construction of Polyploids and Genome Shuffling

Author: DingWenTao
Tutor: MaPingSheng
School: Tianjin University
Course: Biochemistry and Molecular Biology
Keywords: Saccharomyces cerevisiae Ethanol Polyploid Genome rearrangement
CLC: TQ223.122
Type: Master's thesis
Year: 2009
Downloads: 46
Quote: 0
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Abstract


With the deepening understanding of the negative environmental impact caused by fossil fuels , as well as concerns about the depletion of oil resources , has become the development trend of the development of renewable fuels . Bio- fuel ethanol as a clean and renewable energy production and use in many countries . Fuel ethanol production by yeast fermentation of starch or sugar feedstock . High gravity fermentation is a high sugar concentration the emerging ethanol fermentation method to improve the speed of the ethanol concentration and fermentation , thus saving energy consumption , improve equipment utilization . Thick mash fermentation conditions of high osmotic pressure and high concentration of ethanol on the growth and fermentation of the strain is negative , thus increasing the strain in this condition, the fermentation capacity meaningful . In this study, starting from the industrial strain CN1 gravity fermentation conditions of fermentation capacity of its transformation to improve use of two methods : First build polyploid strains investigated genome multiple series of strains of Saccharomyces cerevisiae fermentation capacity differences ; EMS mutagenesis and genome rearrangement screening strains of fermentation capacity . Diploid industrial strain CN1, transformed with a plasmid YCplac33-GHK HO gene expression and induction of the plasmid , the mating type MATa / a and MATα / α diploid strains . CN1 demolition spore mating type MATa and MATα haploid strains . The fusion of different mating type yeast , triploid and tetraploid strains can be obtained . Fermentation experiments showed that , despite polyploid strains of ethanol tolerance improved, but the speed of consumption of sugar and ethanol production was lower than diploid . In this study on the basis of the determined dose of ethyl methane sulfonate (EMS) mutagenesis , the diploid yeast strains CN1 mutagenesis. And take advantage the sporogenes and integration of the yeast , separation and rearrangement of the gene . The end of the high gravity fermentation residual sugar concentration decreased by 21 % , ethanol production increased by 0.8% , the conversion rate increased by 0.5 % ethanol and ethanol tolerance has increased strains WTE1 ; , and residual enrichment, screening and rescreening the sugar concentration reduced by 61% , 1.3% increase in ethanol production and ethanol conversion rate increased by 0.3% strain WTE2 . The study results show that: the strategy used in this study is an effective yeast ethanol fermentation strain transformation method .

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CLC: > Industrial Technology > Chemical Industry > Basic Organic Chemistry Industry > The production of aliphatic compounds ( acyclic compounds) > Aliphatic alcohols (alcohols, hydroxy compounds) and its derivatives > Aliphatic alcohol > Fatty Alcohols > Ethanol ( alcohol )
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