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Hollow Fiber Ultrafiltration Membrane has been widely in all aspects of people’s life and production, such as chemical, environmental protection, electronic, light in- dustry, petroleum, food, pharmaceutical, bioengineering, energy engineering, etc. The performance of Hollow Fiber Ultrafiltration Membrane is concerned for the wide usa- ge of Hollow Fiber Ultrafiltration Membrane. Since there are many methods to chara- cterize the performance of Hollow Fiber Ultrafiltration Membrane, the paper comp- ares these methods.The paper explores the changes of flux under different temperatures and the effects of temperature on the membrane.Compared with two methods of porosity characterization, the deviation of density method is smaller than dry-wet method. In dry-wet method, sample must be immersed in ethanol for a certain period of time before measurement. Membrane may be swell- led making the aperture change because of the occlusion of liquid. Dry-wet method is easy to lead to large error because of more measurement data required. So the porosity got by density method is more in line with the actual.Membrane pore size and distribution measured by gas adsorption-desorption me- thod, liquid displacement method and liquid velocity method is not close to the results measured by FESEM method. The result of DSC method is close to the FESEM me- thod. The surface of membrane is made wet before measurement, which does not in- fluence the structure of membrane pore. So DSC method is an ideal method to mea- sure the membrane pore.Dextran T10 and T40, Polyethylene Glycol 6K, 10K, and 20k, Bovine Serum Albumin, chicken ovalbumin are used to characterize the retention performance of Hollow Fiber Ultrafiltration Membrane. To the marker of the same molecular weight, the retention rate of protein is highest with good reproducibility, followed by PEG, Dextran the worst. Protein molecule itself is spherical molecule with good repro- ducibility and high retention rate. So protein is suggested to characterize the retention performance of Hollow Fiber Ultrafiltration Membrane. Because of the adsorption of membrane material, membrane material has certain effect on the retention per- formance of membrane.
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