Dissertation > Excellent graduate degree dissertation topics show

Genetic Variations of LHX4, LMF1 Gene and Their Associations with Growth Traits in Four Chinese Cattle Breed

Author: RenGang
Tutor: ChenHong
School: Northwest University of Science and Technology
Course: Genetics
Keywords: Cow Candidate genes Polymorphism Production traits
CLC: S823
Type: Master's thesis
Year: 2009
Downloads: 45
Quote: 2
Read: Download Dissertation

Abstract


In this study, using PCR-RFLP, PCR-SSCP and DNA sequencing technology to detect the Nanyang cattle the Qinchuan Jiaxian Red Holstein four groups of 822 individuals LHX4, LMF1 genetic variants, analysis polymorphic loci on the genetic structure and genetic diversity, and polymorphism and growth traits of Nanyang cattle, Qinchuan and jiaxian, Red Bull 3 groups in the loci correlation analysis to test these genes The three cattle population genetic effects of growth and development, in order to find the significant effect of the genetic markers for economically important traits, germplasm conservation and utilization of genetics basis for the establishment of efficient cattle breeding and molecular markers database. This study to obtain the following results: 1 four cattle breeds LHX4 gene polymorphism were detected 3 LHX4 gene locus E1, E3, E6, contains the exon 1 and exon 3 and the outer exon 6. 8 SNPs (NW 0 01,493,442.2: g.35143 G gt; were detected in E3 and E6 locus A, 35152 C gt; T, 35212 C gt; T, 35.23 thousand G gt; A 35233 T gt; C, 45233 C gt; T, 45521 A gt; G, 45530 G gt; A), all located in the coding region, including 45530 G gt; A mutation causes lead to the location of a Hae Ⅲ restriction sites disappear digested after three with type: GG, GA and AA genotype. Various groups in the E3, E6 loci are in Hardy-Weinberg equilibrium, the E3 locus mutation sites polymorphic information content is less than 0.25, a moderate polymorphism in the E6 locus, the Nanyang cattle and Jiaxian Red groups. Jiaxian Red groups E6 Hae Ⅲ site only a moderate polymorphism. The genotype distribution of the chi-square test of independence: relatively large differences between different cattle breeds. The E3 locus Qinchuan and China Holstein Jiaxian Red Nanyang cattle difference not significant outside, other varieties are highly significant difference (P lt; 0.01). E6 locus Jiaxian Red the Nanyang cattle difference not significant, and the remaining varieties are highly significant difference (P lt; 0.05). Chinese Holstein cattle and local cattle difference in E6 Hae Ⅲ site highly significant (P lt; 0.01). 2. LHX4 gene polymorphism and growth traits in Nanyang cattle, Qinchuan and jiaxian, Red Bull, correlation analysis E3 gene, Block: the Nanyang cattle population 18-month-old individual weight MM type is significantly greater than the MN-type (P lt; 0.05) ; E6 loci: the Nanyang cattle groups of 12-month-old girth AA genotype was significantly greater than AC (P lt; 0.05); Qinchuan cattle AB type 4-month-old waist angle width significantly greater than AA-type (P lt; 0.05) . In E6 Hae Ⅲ site: Nanyang the cattle groups of 18 months old individual body length GA type was significantly greater than the AA genotype (P lt; 0.05), and 24-month-old body plagioclase GA type is significantly larger than the AA genotype (P lt; 0.05). Jiaxian Red groups the GA type hucklebone wide significantly larger than that of AA-type (P lt; 0.05) LMF1 gene polymorphism were detected LMF1 the gene E1 E 10 and I7 total of 11 loci . Results: E2, E3, E4, E6, E8 and E9 loci found polymorphism. Detected a total of 18 SNPs and a single base deletion (NC 0 07326.3:6290 C gt; A, 10157 C gt; A, 10230 T gt; A, 27046 G gt; C, 27 103 C gt; T, 27120 A gt; G, 27154 T gt; C, 27224 T gt; C, 39799 C gt; T, 39848 G gt; A, 39871 C gt; T, 50245 C gt; G, 50358 C gt ; T and 50423 G gt; A, 50544 T gt; C, 50583 T gt; C, 50593 C gt; A and 50681 G gt; absence of the A and C bases), of which 27 154 T gt; the C mutations lead to LMF1 protein the 197T gt; A change, including 39848 G gt; to A mutation lead LMF1 protein 289V gt; L changes. Wherein 27154 T GT; C, 50358 C gt; T, 50583 T GT; C, 50593 C gt; the change of A leads to the generation or deletion of the AvaI restriction sites; 39871 C GT; cause the change of T Hin6I restriction sites generation. E4 Ava? Sites: four groups were in Hardy-Weinberg equilibrium. Nanyang cattle, cattle, Jiaxian Red and Chinese Holstein cattle genotype distribution of the four groups of the chi-square test for independence, the differences between the different varieties were significant (P lt; 0.05) or highly significant (P lt; 0.01). E9-83 Ava? Sites: four groups were in Hardy-Weinberg equilibrium. The genotype distribution of the chi-square test of independence: In addition to the the Nanyang cattle Jiaxian Red no significant difference, the rest are highly significant difference (P lt; 0.01). E9-93 Ava? Sites: the groups are in Hardy-Weinberg equilibrium. The genotype distribution of the chi-square test of independence: In addition to the the Nanyang cattle Jiaxian Red, Qinchuan and Holstein were no significant differences, the rest are highly significant difference (P lt; 0.01). Nanyang cattle population in the E2 the E4D sites: in the Hardy-Weinberg equilibrium, polymorphic information content greater than 0.25, belongs to the moderate polymorphism. In E3, E4, E6, E6H and E8A in Hardy-Weinberg non-equilibrium state. Of 4. LMF1 gene polymorphism and growth traits in Nanyang cattle, Qinchuan and jiaxian, Red Bull correlation analysis E9-93 Ava? Sites, the the Nanyang cattle population CA type girth 6 months of age was significantly greater than CC genotype ( P lt; 0.05), 6 1 2 months average daily gain CA type was significantly greater than the CC genotype (P lt; 0.05) Jiaxian Red groups CA lumbar angle width and girth significantly greater than the CC genotype (P lt; 0.05). E2 loci: Nanyang cattle groups 6 months old individual body CT genotype was significantly greater than the CC genotype (P lt; 0.05), and 24-month weight CT genotype was significantly greater than the TT genotype (P lt; 0.05). E3 loci: the Nanyang cattle population 18-month-old body weight of H1H1 in H1H2 type significantly greater than the H2H3 type (P lt; 0.05), and 24-month weight, body length, chest circumference and 19 2 4 months Age average daily gain the H1H2 type significantly greater than the H1H1 type (P lt; 0.05). The E4 locus: MN type of Nanyang cattle groups of 12-month-old individual weight significantly greater than the MM genotype (P lt; 0.05), 24-month weight and girth MN type is significantly larger than the MM genotype (P lt; 0.05). The E4D sites: the Nanyang cattle population OM type in the 12-month-old body weight was significantly greater than the MM and OO type (P lt; 0.05) in the 24-month weight and girth traits OM significantly greater than the MM type (P lt; 0.05).

Related Dissertations

  1. CYP2C19 Gene Polymorphism in Patients with Ischemic Cerebrovascular,R743
  2. Polymorphisms in the Promoter Region of Swine BMP7 Gene and Their Association with Reproductive Traits,S828
  3. Studies on Polymorphisms on Exon and Intron of BMP7 Gene and Their Associations with Reproductive Traits in Pigs,S828
  4. Effects of 2-Hydroxy-4-Methionine Isopropyl on Rumen Fermentation and Production Performance of Holstein Cows,S823.8
  5. Cloning, Expression and Polymorphism Analysis of BMP4 Gene in Hu Sheep,S826
  6. Dendrobium candidum chloroplast microsatellite development and application of interspecific,S567.239
  7. Study on Genetic Variation and Its Effects and Expression of CFL2 Gene in Chickens,S831
  8. Establishment of Different Shell Color Breed Lines of Chlamys Nobilis and Their Development,S968.3
  9. Fine Mapping of Two Leaf Mutant Genes al and yl in Maize,S513
  10. Study on the mRNA Expression Level and the Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Taihu Pig’s Ovary,S828
  11. Epidemiological Investigation of Brucellosis in Livestock and Molecule Typing of Brucella Based on Single Nucleotide Polymorphism (SNP) Analysis,S855.12
  12. Polymorphisms of Y-STRs in Uygur and Kazak Ethnic in Xinjiang,R394
  13. The Relationship of Polymorphisms of Methionine Synthase Reductase(MTRR) Gene and Plasma Homocysteine Levels with Myocardial Infarction,R542.22
  14. The liberation of cattle Network,G206
  15. Promoter Activity Analysis of Cytochrome P450 Gene CYP9A17v2 from Helicoverpa Armigera (H(?)bner),S435.622
  16. Study on the Resistance of IRF-1 Transfected Bovine Fetal Fibroblast Cells to BVDV and IBRV,S823
  17. Study on the Physiological Differentiation and Genetic Diversity of Exserohilum Turcicum in Northeastern China,S435.131
  18. Application of Phytosterols and Its Mechanisms in Dairy Cows,S823
  19. Investigation of the Methods of Drug Using in Dairy Cow Farm in China,S858.23
  20. The Phenomenon That Antibodies Were Activated by Non-corresponding Antigen in Immune Response and the Correlations with H2-Eb Polymorphism,R392
  21. Association of Single Nucleotide Polymorphisms in SCN5A and minK Gene with Atrial Fibrillation,R541.75

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Cow
© 2012 www.DissertationTopic.Net  Mobile