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Honey locust (Gleditsia sinensis Lam.) Leguminous deciduous tree, widely distributed in China, and its pods, seeds and thorns have different medicinal value, at present, on the acacia's research focuses on the identification and separation of saponin extracts clinical efficacy, rather its active ingredients active triterpenoid saponins monomer rarely reported, this paper acacia fruit saponins were isolated and toxicity studies, to obtain the following results: 1. acacia in saponins Isolation and identification: A 70% ethanol percolation extraction, n-butanol, atmospheric combination of silica gel column chromatography, thin layer chromatography repeatedly from acacia fruit isolated one contains seven glycosylated triterpenoid saponins by TLC, mp, IR, MS, 1H-NMR and 13C-NMR analysis method to determine the 3-O-β-D-xylopyranosyl (-1 → 2)-α-L-arabinopyranosyl (-1 → 6)-β- D-glucopyranosyl oleanolic acid 28-O-β-D-xylopyranosyl (-1 → 3)-β-D-xylopyranosyl - (1 → 4)-α-L- rhamnopyranosyl - (1 → 2) - [(6S, 2E) -6 - hydroxy-2,6 - dimethyl -2,7 - oct-ol - (1 → 6)]-β- D-glucose esters South topiramate, namely gleditsioside A. 2 Gleditsioside A toxicity test in mice: oral gavage, the measured Gleditsioside A median lethal dose in mice (LD50) was 508.31mg/kg, 95% confidence interval 449.40mg/kg ~ 574.95mg/kg. By oral gavage for a period of 30d subacute toxicity tests on test mice were daily observations, body weight, blood, blood biochemical and histopathological determination of observation, comprehensive analysis, Gleditsioside A on liver, kidney, spleen and lungs have some damage. 3 acacia Optimization of Extraction Process of Total Saponins and Determination: The vanillin - sulfuric acid colorimetric method to isolate purified from gleditsioside A as reference, the establishment of spectrophotometric determination of total saponin content in acacia; investigate methanol concentration , extraction time, extraction times on the yield of total saponins acacia impact and use orthogonal experiment to optimize their process conditions; measuring 6 to 11 months in total saponins of acacia. The results show that, at a concentration of 0 ~ 164.64μg/mL within the linear range, regression equation was A = 0.0019C-0.0020, R = 0.9993 (n = 7); three factors on the extraction rate of the order: the number of extraction gt; methanol concentration gt; extraction time, extraction of total saponins acacia optimum process parameters: 75% methanol extract 60min, extracted two times; 6,7,8,9,10,11 acacia month total saponins contents were 13.60 %, 14.67%, 15.25%, 22.96%, 20.17% and 15.82%, of which in September acacia highest content of total saponins, followed in October, the lowest in June.
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