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The Study of Resistant Mechanisms to Soybean Cyst Nematode
Author: LiuShuXia
Tutor: XuMei；LiHaiYan
School: Heilongjiang Bayi Agricultural University
Course: Plant Pathology
Keywords: Soybean cyst nematode Cyst Root exudates Conductivity Chitinase
CLC: S435.651
Type: Master's thesis
Year: 2009
Downloads: 15
Quote: 0
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Abstract
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Soybean Cyst Nematode (Heterodera glycines Ichinohe) is the worldwide soybean production in the most serious harm to the soil-borne parasitic disease, after infection, it is extremely difficult to control. The use of resistant varieties is the most cost-effective control measures currently used. In this paper, different resistant varieties, through laboratory and field tests, the soybean cyst nematode cyst hatching and induced resistance preliminary study. The test results are as follows: clear the freshness of the cyst direct impact on the time and the number of hatched nematodes, the time required by the fresh ripe cyst hatched nematode number. In pH 1.0,2.0 and 13.0, the wintering the brown cyst and fresh mature white cyst not occur incubator. 2 the brown cyst under in vitro conditions, whether it is fresh the mature cyst or winter in the field of soil temperature (ie outdoor temperature conditions), 0 ° C, 35 ° C and 45 ℃, the the nematode number of hatched phase for test set temperature 4 ° C, 16 ° C, the indoor temperature is 24 ℃, a small number of hatched nematodes showed a significant downward trend. Processing vitro cyst found different temperatures, as the treatment time, the downward trend in the number of nematodes hatched. Different temperatures processing soil HETERODERA found relative Heterodera under in vitro conditions, suffered little influence of the temperature and time. 3 different resistant varieties roots cyst nematodes amount of resistance relationship hatched research results show that: the number of second-generation cyst susceptible cultivar plants, hatching the number of nematodes. The number of resistant varieties on the second generation cyst is small, the number of nematodes hatched. 4 different varieties of soybean root exudates results show that the influence of the soybean cyst nematode cyst hatching rate: root exudates of susceptible varieties of soybean cyst nematode cyst hatching has a stimulating effect, disease-resistant varieties of root exudates on soybean cyst nematode cyst hatching a certain extent. 5 different varieties of soybean root worm online measurement results show that the conductivity of suspension: different varieties of soybean seedlings in the nematode suspension conductivity with increasing immersion time, susceptible varieties of increase than the resistant varieties , the size of the electrical conductivity can be as soybean resistant cyst nematode resistance evaluation criteria used to determine soybean resistance to soybean cyst nematode resistance strength. The laboratory test results show that: after the inoculation of soybean cyst nematode, after chitin, chitosan, oligosaccharides induced soybean root tissue chitin enzyme activity increased the most obvious, which induced chitin activity peak in advance, activity increased amplitude. Field experiments showed that: chitosan, oligosaccharides and chitin IRRIGATING and coating induced by treatment of soybean, soybean cyst number of per plant were decreased trend. Chitin induction treatment is better than the processing of oligosaccharides and chitosan. Resistance to soybean cyst nematode by coating the seed treatment effect IRRIGATING processing and especially susceptible cultivar effect was more significant.
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CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Economic crop pests and diseases > Oil Crop pests and diseases > Soybean pests and diseases
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