Dissertation > Excellent graduate degree dissertation topics show

Study on the Expression of Transcription Factor T-bet, GATA-3, RORgammat and FOXP3 in PBMC of HCC Patients

Author: WuLiXuan
Tutor: LiuXiaoPing
School: Shantou University
Course: Surgery
Keywords: CD4~+CD25~+T cells Transcription factor T-bet GATA-3 RORgammat FOXP3 HCC
CLC: R735.7
Type: Master's thesis
Year: 2009
Downloads: 109
Quote: 0
Read: Download Dissertation

Abstract


Objective: 1. To investigate whether HCC patients existing Treg advantage situation. 2. To study the expression of transcription factor T-bet, GATA-3, RORgammat and FOXP3 in PBMC of HCC patients.Methods: The proportion of CD4~+CD25~+T cells in the peripheral blood mononuclear cells was determined by flow cytometry; the changes of T-bet, GATA-3, RORgammat and FOXP3 mRNA levels in the peripheral blood mononuclear cells were determined by real-time quantita-tive PCR.Result: The proportion of CD4~+CD25~+T in the peripheral blood mononuclear cells of HCC pa-tients was significantly higher than control group( 17.72±4.97 VS 9.22±2.68, P<0.01 ). the ex-pression of FOXP3 mRNA in HCC patients was higher than healthy control group( 3.17±1.59 VS 1.39±1.13, P<0.01 ), and Spearman Correlation Analysis revealed that FOXP3 mRNA was positively correlated with CD4~+CD25~+T cells percentage in PBMC( HCC Group r=0.761, P<0.01 ). The results of real-time quantitative PCR indicated T-bet mRNA in HCC group was significantly lower than control group( 52.34±34.07 VS 104.±56.00, P<0.01 ), but GATA-3 mRNA in HCC group was significantly higher than control group( 1.38±1.15 VS 0.58±0.65, P<0.05 ), and the T-bet mRNA/GATA-3 mRNA ratio was manifestly reduced in HCC group than control group( 86.01±116.71 VS 461.88±708.81, P<0.05 ). the RORgammat mRNA in PBMC of HCC patients was higher than control group( 72.32±32.82 VS 33.07±22.86, P<0.01 ). Conclusion:1. The ratio of CD4~+CD25~+T cells and the expression level of FOXP3 mRNA was increased in the PBMC of HCC patients. There was a positive correlation between FOXP3 mRNA and CD4~+CD25~+T cells. It suggested the Treg advantage situation in HCC patients. 2. The T-bet mRNA expression in liver cancer patients was depressed than those of control group, while the GATA-3 mRNA was significantly increased, and the ratio of T-bet mRNA/GATA-3 mRNA was decreased. It showed the Th1/Th2 imbalance in HCC patients, and it may be one of the mechanisms of tumor immune escape. 3. The transcription factor RORgammat mRNA of th17 was increased in PBMC of HCC patients, it suggested the Th17 advantage situation in HCC patients.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Cloning Andanalysis of NAC Transcription Factor Gene in Tobacco(Nicotiana Tabacum L.),Q943.2
  3. Molecular Clonging and Functional Analysis of Heat Shock Transcriptional Factor CarHSFB2 in Chickpea (Cicer Arietinum L.),Q943.2
  4. Expressive Characterization of Transcription Factors in Cotton Fibre Initiation and Cloning and Characterization of Three Transcription Factor Genes in Gossypium Hirsutum L.,Q943
  5. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  6. TLR4 Dependent Signaling Pathway in Lipopolysaccharide Induced Human Lung Carcinoma Cells Proliferation,R734.2
  7. Molecular Cloning, Sequence nad Expression Analyses of Two Transcription Factors ZmC4HC3 and ZmNAC,S513
  8. Expression and Their Significance of Apoptosis Regulatory Factors Survivin、XIAP and c-Jun、NF-κB in Epithelial Neoplasm of Ovary,R737.31
  9. Bone marrow mesenchymal stem cells in the same kind of heart transplantation on Treg cells in rats,R654.2
  10. SIRNA interfere with transcription factor SOX2 and OCT4 on the proliferation of cancer stem cells,R735.2
  11. Microplate-nucleotide Hybridization Based Elisa-like Assay for Detection of DNA Binding Protein,Q503
  12. The Influence of Ambroxol and Varenicline Tartrate on Expression of TGF-β1 and IκBα in Rats with Chronic Bronchitis Induced by Smoking,R562.21
  13. Rhizoma Coptidis Inhibits LPS-induced MCP-1 Production in Murine Macrophages,R285
  14. The Isolation and Genetic Transformation of WRKY Gene in Wheat(Triticum Aestivum L.),S512.1
  15. Study on the Role of Osterix in the Heterotopic Ossification of Fluoride Bone Injury,R114
  16. A Preliminary Investigation of the Expression of SK4 in Hepatocellular Carcinoma,R735.7
  17. The Impact of HBV on Oval Cells and Its Effects on the Expression of P8,R735.7
  18. Expression and Clinical Significance of SK4 in Hepatocellular Carcinoma, Breast Carcinoma and Thyroid Carcinoma,R730.4
  19. Tumor cells to CD4 ~ Foxp3 ~ regulatory T cells affect the clinical and experimental studies,R392
  20. Molecular Cloning, Expression Analysis of Defence Related Genes from Citrus Infected by Ca. Liberibacter Asiaticus,S436.66
  21. Influence of Synuclein-γ on the Effectiveness of Antimicrotubule Drugs in Human Hepatoma Cells and the Mechanism,R735.7

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile