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Objective: To investigate the effect of the Shenshuaixiezhuo pill on CTGF,α-SMA of kidney tissue of renal interstitial fribrosis rats caused by unilateral ureteral obstruction (UUO), and to illustrate the mechanism of Shenshuaixiezhuo Pill on delaying renal interstitial fibrosis.Methods: In the experiment,the rats model with renal interstitial fibrosis caused by unilateral ureteral obstruction was adopted.The rats were divided into six groups,including sham-operated group,model control,high-dose SSXZW,middle-dose SSXZW,low-dose SSXZW,and Benazepril group.We detected rat serum creatinines(Scr),blood urea nitrogen(BUN) and 24h urinary protein excretion.HE and Masson staining method were used to observe the pathologic change of the experimental rats’kidney tissues.CTGF andα-SMA expression in kidney tissues were detected by immunohistochemical method.Results: Shenshuaixiezhuo pill can decrease Scr, BUN; Shenshuaixiezhuo pill can significantly lower expression of CTGF andα-SMA in tubulointerstitial and have obvious difference with control group.Shenshuaixiezhuo pill can improve the renal tubulointerstitial pathological lesions.Conclusions: Shenshuaixiezhuo pill can decrease Scr and BUN, so that it can improve renal function. Shenshuaixiezhuo pill can significantly lower expression of CTGF andα-SMA in tubulointerstitial of the unilateral ureteral obstruction animal model.It can reduce ECM sedimentation, improve its pathological lesions, protect renal function and relieve renal interstitial fibrosis.
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