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The Proliferative and Apoptotic Effects of Oxytropis Falcate Bunge and Nux Vomcia Extracts on Cancer Cells

Author: ZhaoYing
Tutor: ChenShuLin
School: Northwest University of Science and Technology
Course: Neurobiology
Keywords: Oxytropis falcate Bunge extracts Nux vomica extracts proliferation inhibitory apoptosis
CLC: R285.5
Type: Master's thesis
Year: 2009
Downloads: 44
Quote: 0
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Abstract


Oxytropis falcate Bunge has an extensive application possibility in the anti-tumor clinical medicine. However, this study was just focused on the flavonoids, and the mechanisms of the anti-tumor activity are still not well understood. Nowadays considerable research efforts have been made to evaluate the anti-tumor effects of Nux vomica. Whereas differents processed manner could influence chemical composition and toxicity of Nux vomica. In this study, Nux vomica was been processed by traditional Tibetan method, which haven’t been reported before. MTT assay , Hoechst 33258 staining and Annexin-V/PI double-staining assay were used to evaluate the effects of proliferation inhibitory and determine the cell apoptosis rate of three cancer cells in vitro.1. Assessment of cell viability by MTT assay shows that Oxytropis falcate Bunge extracts (500μg/mL) exhibited the strongest cytotoxic effect on Smmc-7721 cells at 72h after treatment (67.01%), whereas the maximal growth inhibitory on A549 cells was 25.89% and on C6 cells was 74.61% . All kinds of cells died in a time and dose-dependent manner. After incubation with Nux vomica extracts for 72h(500μg/mL), the cells inhibitory rate of Smmc-7721 cells was 60.33% . And maximal growth inhibitory on A549 cells was 53.73% and on C6 cells was 74.61%.2. In assessment of cell apoptosis by Annexin V and PI double staining, the mean apoptotic population of Smmc-7721 cells was 3.20±0.26% under control conditions, while it was increased to 12.03±3.26% after treatment with 250μg/mL Oxytropis falcate Bunge extracts for 48 h. At the same time, A549 cells and C6 cells were increased to12.67±6.03% and 13.23±7.61% respectively. The results presented in Nux vomica extracts showed that after treatment for 48 h, the apoptotic rates Smmc-7721 cells, A549 cells and C6 cells were increased markedly reached 11.07±4.61%, 13.50±3.92% and 13.90±4.12% respectively.3. Hoechst 33258 staining showed that there were significant morphological changes in the nuclear chromatin after incubation with Oxytropis falcate Bunge extracts (500μg/mL) and Nux vomica extracts (500μg/mL) for 48 h. In the untreated group, the nuclei were stained a less bright blue and the color was homogeneous. After treating with Oxytropis falcate Bunge extracts (500μg/mL) and Nux vomica extracts (500μg/mL) for 48 h, the blue emission light in apoptotic cells was much bright than the control cells. Condensed chromatin could also be found in many treated cells.and some of them formed the structure of apoptotic bodies,which is one of the classic characteristics of apoptotic cells.From the results above, we draw the following conclusions: the anti-tumor activity of Oxytropis falcate Bunge extracts and Nux vomica extracts . The activity could be partially attributed to the induction of apoptotic cell death .The results offered the evidence for studying anti-tumor drug.

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