Dissertation > Excellent graduate degree dissertation topics show

Interspectific Hybridization between Chrysanthemum Species and Research on Resistant Germplasm Innovation

Author: ChengZuo
Tutor: ChenFaZuo
School: Nanjing Agricultural College
Course: Ornamental Plants and Horticulture
Keywords: Chrysanthemum grandiflorum C. nankingense C. makinoi Interspecific hybridization Backcross Resistance Germplasm innovation
CLC: S682.11
Type: Master's thesis
Year: 2010
Downloads: 19
Quote: 0
Read: Download Dissertation


Chrysanthemum(Chrysanthemum grandiflorum (Ramat.) Tzvel.) is one of the most important ornamentals, enjoying a major share of the cut flower and flowering pot plant market worldwide. However, the majority of chrysanthemum cultivars are susceptible to a range of diseases, pests and abiotic stresses. Many Chrysanthemum wild species have been recognized levels of tolerance to various biotic or abiotic stresses, and interspecific hybridization offers opportunities to transfer useful genetic variation to elite germplasm. Here, in order to introduce excellent traits from C. nankingense and C. makinoi to commercial cultivar Chrysanthemum grandiflorum’rm20-12’, we produced F1 progeny between them. Then, to improve the ornamental value of F1 hybrid of C. makinoi x’rm20-12’, we produced BC1F1 progeny using’rm20-12’as the recurrent parent. We surveyed the morphology, cold tolerance, aphid resistance and heat tolerance of these hybrid progeny. The main results and conclusion are listed as follows:(1) Ovary rescue was employed to create six interspecific hybrids from the cross between Chrysanthemum grandiflorum (Ramat.) Tzvel.’rm20-12’(2n=54) and its wild diploid relative C. nankingense (Nakai) Tzvel. (2n=18). The morphology of the hybrids differed from that of either parent. The leaf length and width of all three C. grandiflorum x C. nankingense hybrids exceeded that of the parents, as did the plant height of two and the inflorescence diameter of another of the hybrids. The RN1 plant was tall (66.88 cm), with long, wide leaves (6.18 cm and 3.99 cm, respectively). RN2 had the longest leaves (8.73 cm), widest leaves (5.62 cm) and largest inflorescence diameter (5.06 cm), while RN3 had a leaf length of 5.68 cm and a leaf width of 4.73 cm. One of the reciprocal hybrids NR2 was heterotic for leaf length and width (6.55 cm and 4.40 cm). All the hybrids bore yellow flowers. The cold tolerance of five hybrids was significantly superior to that of their C.×grandiflorum parent for the LT50S of the five hybrids lay between-15.93℃and-21.23℃, significantly lower than that of C. grandiflorum-14.33℃. C. nankingense appears to be a suitable donor to C. grandiflorum for cold tolerance. Interspecific hybridization clearly provides an effective means of cultivar improvement in chrysanthemum.(2) Three backcross hybrids using C. grandiflorum’rm20-12’as paternal parent was obtaine, and the morphology of them differed from that of both parents NR2 and’rm20-12’ NR2R1 was tallest (64.55cm), and the inflorescence of NR2R3 developed most tubular florets, compared to their parents. The other characteristcs of hybrids were intermediate between two parents, or the same as one of the parents. The flower color in BC1F1 hybrids was orange and red, while NR2 bore yellow flowers and’rm20-12’bore red flowers. The cold tolerance of three BC1F1 hybrids was significantly superior to that of their C. grandiflorum parent for the LT50S of the three hybrids lay between-17.31℃and-19.06℃, significantly lower than that of C. grandiflorum-14.33℃. Backcross provides an effective means to improving ornamental value and cold torance of F1 hybrid.(3) The interspecific cross between Chrysanthemum grandiflorum (Ramat.) Tzvel. ’rm20-12’(R,2n=54) and C. makinoi Matsum.& Nakai (M,2n=18) was achieved using embryo rescue, and a single backcross progeny using C. grandiflorum ’rm20-12’ as paternal parent was obtained. The morphology of the two independent F1 hybrids (RM1 and RM2) differed from that of both parents. RM1 had a larger inflorescence diameter (4.9cm) along with narrow leaves (2.2cm) and a reduced number of ray and tubular florets (17.5 and 144.5). RM2 was shorter (30.8cm) and its inflorescence developed fewer tubular florets (148.8) than either M or R. The BC1F1 hybrid was similar to its maternal plant RM2R in terms of leaf length and width, inflorescence diameter and the number of ray florets, while it produced fewer tubular florets (103.9) than either RM2 or R. The flower color in both F1 hybrids was red-purple (red-purple group N74A and N74B), while the BC1F1 plant bore red flowers (red group 55A). The aphid resistance and heat tolerance of RM1, RM2 and the BC1F1 hybrid were both significantly superior to that of C.×grandiflorum ’rm20-12’. Backcrossing can maximize the recovery of the elite traits. Interspecific hybridization followed by backcrossing shows clear potential for cultivar improvement in chrysanthemum.

Related Dissertations

  1. Study on Properties of Thermal Shock and Ablation Resistance for Gr/Al-Mg Composites,TB332
  2. Research on Temprature Controling Technology of Laser Diode with Thermoelectric Cooler,TN248.4
  3. The Study on Arabidopsis Thaliana Heat Shock Factor HSFA 1d Response to Formaldehyde Stress,Q945.78
  4. Cultivation and Effects of Environmental Factors on Carrying WSSV Virus of Litopenaeus Vannamei,S945
  5. Design, Synthesis and Biological Evaluation of Novel Podophyllotoxin Derivatives as Antitumor Agents,R284
  6. Study on Relationship between Endothelin-1, Insulin Resistance and Acute Ischemic Cerebrovascular Disease,R743.3
  7. Experimental study of roadway flexible material the wind resistance of the air curtain group,TD724
  8. Research and Development of Wear Resistance for Physically Disabled Men Jackets,TS941.2
  9. Monitoring of Insecticide Resistance and Resistance Risk Assessment for JS118 in the Rice Stem Borer, Chilo Suppressalis (Walker),S435.112.1
  10. An Approach for Identifying a Plant Resistance Gene Based on the Random Forest,Q943
  11. Situ synthesis of mullite stone whisker toughening microwave metallurgical corundum - mullite Refractories,TQ175.6
  12. The Study of Yeast Frost-resistance and Application in Frozen Dough,TS213.2
  13. Study on the Resistant Mechanism of Japanese Foxtail (Alopecurus Japonicus Steud.) to Haloxyfop-R-Methyl,S451.2
  14. The Resistance and Resistance Chemical Control of Japanese Foxtail (Alopecurus Japonicus Steud.) to Haloxyfop-R-Methyl and Its Cross Resistance, Multiple Resistance with Different Herbicides,S451.2
  15. Using an F2 Screen to Estimate the Frequency of Resistance Alleles to Cry1Ab in Wenzhou Population of Chilo Suppressalis and Cloning of an Aminopeptidase N Gene CsAPN3,S435.112.1
  16. Assessment of Resistance Risk in Spodoptera Exigua (Hubner) and Spodoptera Litura (Lepidoptera: Noctuidae) to Chlorantraniliprole,S433
  17. Study on Resistance of American Sloughgrass (Beckmannia Syzigachne (Steud.) Fernald) Population in Wheat Field to Fenoxaprop-P-Ethyl,S451.221
  18. Effect of Planting Densities and Modes on Lodging Resistance of Stem in Summer Maize,S513
  19. Transformation of RdreBlBI Gene Drived by Rd29A in ’Benihope’ Strawberry,S668.4
  20. Incidence and Mechanisms of Insecticides Resistance to Pyrethroids and Endosulfan in Colorado Potato Beetle, Leptinotarsa Decemlineata (Say),S435.32
  21. Incidence and Mechanisms of Insecticide Resistances to Organophosphates and Carbamates in Colorado Potato Beetle, Leptinotarsa Decemlineata (Say),S435.32

CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Perennial Flowers Class > Chrysanthemum
© 2012 www.DissertationTopic.Net  Mobile