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The Preparation of Targeting SPIO and Its Application in MRI

Author: ZhangLinZuo
Tutor: WuQiuFang；ChenXueMei
School: East China University of Science and Technology
Course: Chemical Engineering
Keywords: Targeting Co-precipitation Fe3O4 Superparamagnetic MRI
CLC: TB383.1
Type: Master's thesis
Year: 2012
Downloads: 220
Quote: 0
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Abstract


composite nanoparticles coverd by bilayer surfactants were obtained. Preparation process and surface coating of nanoparticles was investigated, and as well as surface of the composite particles activation and then coupled with targeting peptide, at last its application in magnetic resonance imaging was studied.Fe3O4 nanoparticles coated by sodium oleate was obtained by means of co-precipitation method.The influence of temperature, concentration, stirring speed and feeding method on particles size was investigated. Then the paticles was coated by three different coating agents for the second layer, and their coating effect was evaluated. The surface of composite coated by dextran was activated by two different methods, and then coupled with targeting peptide. Its target binding property in cell experiment and its application in MRI as contrast agent. Results showed that:1. In a high speed shearing machine, which stiring speed was 7000r/min, when the temperature is 70℃, the concentration of FeCl is 0.1mol/L, the dosage of sodium oleate was 30% of Fe3O4, Fe3O4 nanoparticles coated by sodium oleat were 8nm in average, single-domain crystal and superparamagnetic, and their saturation magnetization was 51.6A·m2/kg.2. Fe3O4 nanoparticles covered by sodium oleat were coating by dextran, carboxymethyl chitosan, and PEG for the second layer. The composite nanoparitcles which were double coated were spherical and still showed superparamagnetic, and their hydrophilicity, dispersity and their magnetic fluid stability were greatly improved, especially composite nanoparticles covered by dextran had the best performance, compared with Fe3O4 nanoparticles coated by single layer, their hydrodynamic diameter was reduced from 146.6nm to 75.1nm, and zeta potential of magnetic fluid was changed from -18.2mV to -63.3mV.3. Composite nanoparticles covered by dextran were activated and coupled with peptide. After oxidation of dextran on the surface of composite nanoparticels by NaIO4, the dextran which was activated could be coupled with four different polypeptide. As a result, the composite nanoparticles to to be targeted SPIO, and could be accumulated in tumor. Gathering concentration of CT247-Dextran-SPIO is 2.13 times of physiological saline.4. Composite nanoparticles covered by dextran were activated and cross-linked by epoxy chloropropane, and then coupled with pepited to be targeted-CL-SPIO. Their hydrophilicity was increased after cross-linked, thus the half-life of particles in the plasma was extended to 6 hours. They could also gathering in tumor, and concentration of CT247-CL-Dextran-SPIO in the tumor was 4.43 times of physiological saline,3.68 times of no-target-SPIO.

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