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Cigarette is a special food and tobacco flavors this particular food additive, has an integral role in the formation of the cigarette style characteristics, the formula is the core technology in the cigarette process, tobacco flavors and fragrances security has been much concern of consumers and the tobacco companies. Flavors and fragrances added to cigarette toxicity is very worthy of study and discussion. Harmfulness important means of evaluation of cell toxicity and genotoxicity tests as a harmful substance is widely used. In this study, CORESTA (international the tobacco scientific research cooperation center) recommended three in vitro toxicological evaluation methods MTT (tetrazolium salt colorimetry) cytotoxicity test, micronucleus test and AMES test (Salmonella typhimurium reverse mutation experiment) cigarette flavors and fragrances and its split components were evaluated. In this paper, the main contents and results are as follows: 1, flavors and fragrances, and its split component is not added to cigarette before, increasing the concentration of cells with the material survival rates are declining, a dose effect was a negative correlation. Full Flavor cytotoxicity was significantly higher than whole spices (p lt; 0.01) whole spices 10 split component in the maximum amount of reagent (50 ml / L) did not show cytotoxic Full Flavor 10 split component cytotoxic differences, 3 #, 5 #, 7 # 8 # (This number is the number of the split components, the specific composition of trade secrets, this does not give) lower cytotoxicity of perfume components, several flavors cytotoxic component, but the cytotoxicity of all flavors and the split components are significantly lower than the full flavor (p lt; 0.01). 2, flavors and fragrances, and its split component is added to the cigarette, with CSC (cigarette smoke agglutination) increasing the concentration of the cell survival are declining, also showed a dose-response, a negative correlation. Comparison with the control cigarette, to add flavor cigarette, cytotoxicity was no significant increase, compared with the Full Flavor, 10 Flavors split component cytotoxic undifferentiated. Compare with the control cigarette, the cigarette of added flavoring cytotoxic also no significant increase in cytotoxicity compared with whole spices, spice split component 10 undifferentiated. Flavors and Fragrances split components of cigarette smoke CSC micronucleus assay, flow cytometry results showed that cell micronucleus by cigarette smoke exposure is higher than normal cells exposed to full flavor # 10 # split component is slightly higher than the other components of the micronucleus rate, but micronucleus rate of full flavor and add smoke flavor blank no difference. Whole spices 3 #, 4 #, 5 # split component is slightly higher than the other components of the micronucleus rate, but the whole spices micronucleus rate was significantly lower than add spice to the control smoke. Prompted some of the split components of cigarette genetic toxicity, suggesting that use less or no highly toxic components in tobacco flavor compatibility to ensure its security in the application of the cigarette. Flavors and fragrances split components of cigarette smoke CSC mutagenic analysis, the the CSC added amount of the number of mutant colonies was a significant positive correlation, there is a linear dose relationship, that with increasing doses of CSC, the cause The mutation also increased correspondingly. Compared with the control cigarette, full flavor, spices and split components of mutagenicity was no significant difference. Throughout the experiment results suggest that the flavors and fragrances in the cigarette is safe.
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