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Effect of Irradiated and Refluxed Silver Nanoparticles on Enzymatic Activity of Glucose Oxidase

Author: QuYi
Tutor: LuWeiChang
School: East China Normal University
Course: Physical and chemical
Keywords: Silver nanoparticles Glucose oxidase Illumination Reflux
CLC: TB383.1
Type: Master's thesis
Year: 2010
Downloads: 33
Quote: 0
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Abstract


In order to improve the performance of the glucose oxidase electrode, people have tried a variety of materials to fix the glucose oxidase enzyme (GOD). Metal nano-particle surface area, strong adsorption capacity, able to strongly bind to the enzymes and biological molecules and metal nanoparticles also has good biocompatibility, the catalytic efficiency and high metal nanoparticles are widely used enzyme fixed. The research showed that gold nanoparticles (AuNPs) has the ability to obtain electron from the surrounding environment, this ability can be accelerated the GOD catalyzed process in the active center (FAD) of electron transfer rate, which play an enhanced activity. Compared with AuNPs silver nanoparticles (AgNPs) GOD activity complex. Through the AgNPs the light reflux treatment is intended to explore the impact of AgNPs GOD activity. Illumination AgNPs GOD activity. The AgNPs with GOD the role of the different illumination time the formation of the the GOD-AgNPs heterogeneity body to study the GOD-AgNPs heterogeneity activity changes. Illumination AgNPs can inhibit the activity of the GOD. More illumination, AgNPs GOD activity inhibition. The experiment makes use of the UV-vis absorption spectroscopy, Zeta-potential, TEM and XPS were characterized by means of the light of AgNPs track. The experimental results show that the light can change the surface of the composition and structure of the AgNPs. The increase of Ag concentration can inhibit the activity of the GOD, AgNPs surface structure changes also affect fixed to AgNPs GOD conformational changes. Preliminary study of of AgNPs light GOD activity inhibition. AgNPs reflux GOD activity. It was found that after a short period of time the return of AgNPs inhibit the activity of GOD. Extension of the the AgNPs reflux time AGNPS Inhibition of GOD activity weakened under reflux for a certain time, the AgNPs can enhance GOD activity. The experiment makes use of the UV-vis absorption spectroscopy, DLS characterization means AgNPs reflow process tracking, found that the particle size of AgNPs increases gradually with the prolongation of reflux time. The fixed reflux different time AgNPs different conformational changes of GOD the, GOD-AGNPS heterogeneity activity. GOD biological activity inhibition and enhanced role of AgNPs reflow process. Silver nanocrystals of different morphologies GOD activity. The use of light and heat curing means get the the different morphologies sheet nano silver, including the triangular flaky, hexagonal plate and a disc-shaped nano silver. UV-vis absorption spectroscopy and other characterization methods, to track the formation of silver nanoparticles of different morphologies sheet to explore the formation mechanism of the different morphologies of the sheet of silver nanoparticles. Using an electrochemical method to examine the impact of the of different morphologies sheet Nano Silver GOD activity. The experimental results show that, due to the catalytic ability of H202, the different effects of hexagonal platelets and pellet of nano silver GOD activity.

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