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Preparation and Characterization of Carboxybetaine Zwitterionic Polymer

Author: HeShuMan
Tutor: ZhouJian
School: South China University of Technology
Course: Chemical Engineering
Keywords: carboxybetaine zwitterionic polymers quaternization reaction albumin protein- resistant material
CLC: TQ460.1
Type: Master's thesis
Year: 2010
Downloads: 171
Quote: 0
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Abstract


Protein-resistant materials are very important in applications such as biomedicine, marine coating, biochip etc. It has been a hot research topic. It is found that some zwitterionic polymers possess excellent protein-resistant performance.The molecular structure and synthetic route of one novel carboxybetaine zwitterionic monomer were designed based on structure-activity relationship, in terms of antifouling theory and requirements of the products’application. Taking 3-acrylamido- N,N-dimethypropylamine （DMAPAA） as the raw material, one novel carboxybetaine zwitterionic monomer, 1-Carboxy-N,N-dimethyl-N-（3-acrylamidopropyl） ethanaminium inner salt （CBAA-1）, was obtained by quaternization reaction with sodium chloroacetate （ClCH2COONa）. IR spectral analysis was employed to confirm the structure of the monomer. An optimum reaction condition for the preparation of CBAA-1 monomer was found: water works as the solvent; the molar ratio of DMAPAA and ClCH2COONa is 1:1.05; reaction temperature is 70°C and reaction time is 5 hours, the conversion rate of tertiary amine reaches 98.5%.CBAA-1 zwitterionic monomer was polymerized to obtain carboxybetaine zwitterionic polymers p（CBAA-1） through solution polymerization. IR spectral analysis was used to confirm the structures of the synthesized polymer. Optimum reaction condition to synthesize p（CBAA-1） by static contact angle measurement of p（CBAA-1） was found: the concentration of monomer is 20%, the concentration of initiator is 0.8% of that of the monomer; reaction temperature is 50°C and reaction time is 6 hours, the static contact angle reaches 44°, carboxybetaine zwitterionic polymers p（CBAA-1） show the best hydrophilicity.Surface Plasmon Resonance was used to characterize carboxybetaine zwitterionic polymers p（CBAA-1）’s protein-resistant performance. Both positively-charged and negatively-charged self-assembled monolayers （SAMs） were prepared on the gold surface. Through electrostatic adsorption, carboxybetaine zwitterionic polymers p（CBAA-1） were adsorbed at positively-charged NH2-SAM and negatively-charged COOH-SAM respectively and then decorated with immobilized albumin. SPR instrumentation characterized the adsorption of fibrinogen. The experimental results showed that, p（CBAA-1） adsorbed on negatively-charged COOH-SAMs shows better protein adsorption resistant ability and better ability to immobilize albumin. After the immobilization of albumin, p（CBAA-1）’s protein -resistance ability increases greatly.

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