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Objective To detect the expression levels of osteopontin (OPN) in children with congenital heart disease (CHD) myocardial analysis of OPN with CHD types (cyanotic and non-cyanotic), myocardial fibrosis, hypoxia level, age relationship, to explore the role of OPN in the CHD myocardial fibrosis. Method to select patients with congenital heart disease underwent surgery during the same period 45 cases, including 25 cases of non-cyanotic congenital heart disease (non-cyanotic group), 15 males and 10 females, aged 0.5-9.2 years old, with an average of 5.03 ± 1.08 years old ; 20 cases of cyanotic congenital heart disease (cyanotic group), 12 males and 8 females, aged 0.5-8.5 years, an average of 4.53 ± 1.82 years. The intraoperative taken right atrial appendage myocardial tissue 1OOmg, and the two groups of children with myocardial type Ⅰ type III collagen, OPN mRNA expression level was detected by reverse transcription - polymerase chain reaction (RT-PCR). Application SPSS13.0 statistical analysis software were compared between the two groups of children with myocardial type Ⅰ type III collagen, OPN expression levels and the purpose of gene and hypoxia, age, defect size, and OPN mRNA and type Ⅰ, Ⅲ type The relationship of the collagen mRNA. Results (1) cyanotic congenital heart disease group than non-cyanotic congenital heart disease group Ⅰ collagen and type Ⅲ collagen mRNA expression levels were significantly increased (type I collagen p lt; 0.01, type III collagen protein p lt; 0.01); (2) cyanotic congenital heart disease group than in the non-cyanotic congenital heart disease group OPN mRNA expression levels were significantly higher (p lt; 0.05); (3) I type Ⅲ collagen expression in children age positively related (type I collagen and children age r = 0.331, p lt; 0.05; type III collagen and children age r = 0.372, p lt; 0.05), with oxygen saturation a negative correlation (type I collagen, and oxygen saturation r = -0.561, p lt; 0.001; type III collagen and oxygen saturation r = -0.537, p lt; 0.001); (4) OPN mRNA expression level and myocardial collagen protein's expression and defect size as is relevant (OPN and type Ⅰ collagen protein r = 0.625, p-lt; 0.001; OPN and type Ⅲ collagen protein r = 0.287, p-lt; 0.05; OPN and defect size r = 0.524 , p lt; 0.001), and oxygen saturation into a negative correlation (the OPN and blood oxygen saturation r = -0.703, p <0.001). Conclusion (1) cyanotic congenital heart disease than non-cyanotic congenital heart disease myocardial collagen type Ⅰ and Ⅲ collagen expression levels were significantly increased; (2) OPN was significantly higher in cyanotic congenital heart disease myocardial positively correlated with indicators of myocardial fibrosis, OPN may be involved in the process of congenital heart disease, cyanotic congenital heart disease, myocardial fibrosis; (3), congenital heart disease, myocardial fibrosis may be with children age, lack of related to the degree of oxygen; (4) of OPN expression and blood oxygen saturation was negatively related, there is a positive correlation with the defect size, prompted increased hypoxia and volume overload can cause OPN expression was enhanced.
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