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The Effect of Chronic Intermittent Hypoxia on Expression of HIF-α, TNF-α, IL-6 in HepG2 Cell

Author: ZuoChang
Tutor: YangYu
School: Central South University
Course: Geriatrics
Keywords: Chronic intermittent hypoxia Chronic continuous hypoxia Hypoxia-inducible factor -1α Interleukin-6 Tumor necrosis factor -α
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 55
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Abstract

Purpose of obstructive sleep apnea-hypopnea syndrome (OSAHS) is a potentially dangerous disease, the incidence of 2% to 4% higher incidence in the elderly. OSAHS with coronary heart disease, hypertension, artery atherosclerosis are closely related. Chronic intermittent hypoxia is one of its main pathogenic mechanisms, studies have found that chronic intermittent hypoxia and oxidative stress, inflammatory response is closely related to. This experiment intends by chronic intermittent hypoxia in human hepatoma cells (HepG2 cells) and chronic continuous hypoxia intervention study chronic intermittent hypoxia and transcription factor HIF-1α relationship of inflammatory cytokines TNF-αIL-6, and preliminary chronic intermittent hypoxia the impact of the inflammatory response, experimental data and theoretical basis for clinical treatment. Method 1. HepG2 cells to establish a cell model, depending on of hypoxia the extent and mode is divided into five groups: 21% of the oxygen concentration in the group; 10% of the intermittent hypoxia group; 5% intermittent hypoxia group; 10% sustained hypoxia group; 5 % intermittent hypoxia group. 2. Laboratory tests: ELISA determination of cell culture supernatant IL-6, TNF-a concentration and the nucleus HIF-1α concentration. Results 1.HIF-1α test results: sustained hypoxia 5% group and the sustained hypoxia 10% group was no significant difference (p gt; 0.05), intermittent hypoxia and intermittent hypoxia 10% 5% group was not statistically difference (p gt; 0.05), intermittent hypoxia 5% group with sustained hypoxia 5% group was significantly higher (p lt; 0.05), 10% of the intermittent hypoxia for 10% of the group was significantly higher (p lt; 0.05); each group compared with 21% oxygen concentration was significantly higher (p lt; 0.05); 2.IL-6 test results: 5% 10% of the group with sustained hypoxia was significantly higher (p lt sustained hypoxia ; 0.05), intermittent hypoxia and intermittent hypoxia 10% 5% group comparison was significantly higher (p lt; 0.05), intermittent hypoxia 5% of the group with sustained hypoxia 5% group was significantly higher (p lt; 0.05) , intermittent hypoxia 10% for 10% of the group was significantly higher (p lt; 0.05) groups compared with 21% oxygen concentration was significantly higher (p lt; 0.05); 3.TNF-α test results: continued 10% of the group with sustained hypoxia hypoxia 5% group was significantly higher (p lt; 0.05), 5% group intermittent hypoxia and intermittent hypoxia 10%, significantly higher (p lt; 0.05), intermittent hypoxia 5 % group with sustained hypoxia group 5% was significantly higher (p lt; 0.05), 10% of intermittent hypoxia with the last 10% of the group was significantly higher (p lt; 0.05) groups compared with 21% oxygen concentration significantly higher (p lt; 0.05); this experiment HIF-1a, IL-6, TNF-a pairwise correlation analysis: HIF-1a and IL-6 comparison was positively correlated (r = 0.287, p, lt; 0.05); HIF-1a and TNF-a was relatively positive correlation (r = 0.273, p lt; 0.05); TNF-a and IL-6 compare to a positive correlation (r = 0.713, p lt; 0.05). Conclusion 1. Chronic intermittent hypoxia with chronic persistent hypoxia can cause human hepatocellular carcinoma cells activate HIF-1α and IL-6, TNF-a expression increased; 2. Chronic intermittent hypoxia on human hepatoma cell TNF-a, IL 6 expression in more obvious than in chronic persistent anoxic environment, chronic sustained hypoxia environment than chronic intermittent hypoxia HIF-1α expression significantly.

The Effect of Chronic Intermittent Hypoxia on Expression of HIF-α, TNF-α, IL-6 in HepG2 Cell

Abstract

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