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5 - aza -2'- deoxycytidine on human cervical cancer cell line HeLa and SiHa cells of Syk abnormal methylation and expression

Author: XuShuYun
Tutor: ZhaoShuPing
School: Qingdao University
Course: Obstetrics and Gynaecology
Keywords: Syk gene HeLa cell lines SiHa cell lines Methylation 5 - aza -2'- deoxycytidine
CLC: R737.33
Type: Master's thesis
Year: 2010
Downloads: 38
Quote: 0
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Abstract


Objective To study different concentrations of demethylation drug 5 - aza - 2' - deoxycytidine on human cervical cancer cell line HeLa and SiHa cells Syk gene promoter region abnormal methylation and expression . Method MSP method to detect cervical cancer cell line HeLa and SiHa cells the methylation status of the promoter region of Syk ; MTT detect different concentrations of 5 - aza - 2'- deoxycytidine (5-Aza-CdR) HeLa cells after the intervention proliferation ; MSP assay with different concentrations of 5 - aza - 2' -deoxycytidine (5-Aza-CdR) HeLa and SiHa cells Syk gene promoter region methylation status changes before and after the intervention ; RT-PCR HeLa and SiHa cells were detected before and after treatment SykmRNA expression level changes . Results exist HeLa cells Syk gene promoter region methylation the SiHa cells Syk gene promoter region and no methylation ; 5 - aza - 2'-deoxycytidine inhibited the growth of HeLa cells , 5 - aza - the 2' - deoxycytidine concentration of 1μM when the best growth inhibition of HeLa cells ; 5 - aza -2'- deoxycytidine on HeLa cells promoter region methylation reversal effect , the drug concentration of 1μM the best of methylation to : enable HeLa cells SykmRNA , the expression level was significantly higher. Conclusion HeLa cells Syk gene promoter region of methyl 5 - aza - 2'- deoxycytidine intervention can make it Syk methylation gene re- expression and inhibit cell growth .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Uterine tumors
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