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Objective: To investigate the relationship of the female population in Shandong province , catechol -O-methyl transferase of COMT cell cycle kinase suppressor gene of P21WAF1 nuclear mitotic apparatus protein NuMA single nucleotide polymorphism with sporadic breast cancer . Method : using allele-specific amplification method (allele specific amplification, ASA) Val158Met COMT gene Shandong Province, 122 cases of normal controls and 140 patients with breast cancer , of P21WAF1 gene Ser31Arg, NuMA the gene Ala794Gly single nucleotide polymorphism detect the differences of the two groups were compared in various genotype distribution frequency , and for statistical analysis. Results: 27.1% ( 38 / 140 ) of the COMT gene in sporadic breast cancer patients and 8.2% ( 10 / 122 ) and normal controls homozygous variation between the two groups Met / Met homozygous genotype frequencies differences were significant significance (x2 = 15.638, P lt; 0.001); With the staging of breast cancer (OR = 5.00, P lt; 0.001), histological grade ( OR = 5.40 , P lt ; 0.001) of elevated and lymph nodes transfer increased (OR = 3.00, P = 0.002), COMT mutation genotype significantly increased the proportion . And 54.3% ( 76 / 140 ) of patients with breast cancer and 42.6% ( 52 / 122 ) of the control 's P21WAF1 gene heterozygous mutation occurs , the difference was not statistically significant ( x2 = 3.549 , P = 0.060) ; 15.7% ( 22 / 140 ) of patients with breast cancer and 16.4% ( 20 / 122 ) and normal controls NuMA gene heterozygous mutation occurs , the difference was not statistically significant ( x2 = 0.020 , P = 0.886 ) . Conclusion : COMT gene Val158Met single nucleotide polymorphism may be related to the occurrence of Shandong Province women with sporadic breast cancer , can be used as an indicator of early genetic diagnosis of breast cancer .
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