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Clinical Exploration on Hematologic Malignancies Treated by Autologous Peripheral Blood Stem Cells

Author: HeZiYan
Tutor: YuanZhong
School: Zunyi Medical College,
Course: Department of Hematology
Keywords: Cryopreservation Autologous peripheral blood stem cells Hematopoietic reconstitution
CLC: R733
Type: Master's thesis
Year: 2010
Downloads: 19
Quote: 0
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Abstract


Objective: To observe the the -80 ℃ direct cryopreserved autologous peripheral blood stem cell (APBSC) activity and observation hematopoietic reconstitution after autologous peripheral blood stem cell transplantation (APBSCT) in the treatment of hematologic malignancies ( malignant lymphoma , acute leukemia ) transplantation . Methods: CS3000 Plus blood cell separator reached after 1-3 times , collecting PBSC transplantation required number range . The stem cells will be collected directly frozen at -80 ℃ . Before freezing , recovery sample to measure the MNC count , CD34 cell count , the trypan blue exclusion dye uptake . And peripheral blood monitoring after transplantation . Results: before freezing MNC Mean ( 4.91 ± 3.03 ) × 108/kg CD34 cells ( 3.32 ± 2.45 ) × 106/kg trypan blue exclusion dye was ( 95.20 ± 1.68 )% ; recovery MNC average ( 4.16 ± 2.55 ) × 108/kg CD34 cells (2.86 ± 1.98) × 106/kg, trypan blue exclusion dye was ( 83.50 ± 2.32) % . MNC, CD34 cell count and trypan blue exclusion dye uptake before freezing and recovery compared were statistically significant ( P lt; 0.05 ) . MNC, CD34 cell recoveries were 84.73% , 86.14% . All patients reconstitute hematopoiesis after transplantation . After transplantation, peripheral blood of the WBC gt; 1.0 × 109 / L , the ANC gt ; 0.5 × 109 / L , PLT gt ; 20 × 109 / L recovery time is 10.80 ± 1.25d 10.40 ± 1.13d , 13.30 ± 4.09d . Conclusion : APBSCT is a convenient and effective treatment of hematologic malignancies . The -80 ℃ direct cryopreservation , PBSC its recovery rate to meet the transplant needs hematopoietic reconstitution after transplantation can be obtained , but some of the effects of freezing effect of interference factors that require further attention .

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms
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