Dissertation > Excellent graduate degree dissertation topics show

Study on Carbon Nanotubes Signal Amplification and High Sensitive Biosensor Construction

Author: ChenHuan
Tutor: WuQi；LinXianFu
School: Zhejiang University
Course: Analytical Chemistry
Keywords: Carbon nanotubes Layer by layer assembly electrostatic interaction biospecific interactions Glucose Carcinoembryonic antigen
CLC: TP212.3
Type: Master's thesis
Year: 2012
Downloads: 65
Quote: 0
Read: Download Dissertation

Abstract


This dissertation focuses on designing and fabricating novel biomimetic interface combining with functional carbon nanotubes (CNTs) for the immobilization of biomlecules. Transmission electro microscopy (TEM), Scanning electron microscopy (SEM), and electrochemical techniques are used to study the properties of the interface. We discussed their applications in bioanalysis. This research focus on the details as follow:1. Bienzyme bionanomultilayer electrode for glucose biosensing was constructed based on polyelectrolyte functional CNTs through layer-by-layer (LBL) assembly. Bionanomultilayer electrode was fabricated based on electrostatic interaction between carbon nanotubes and horseradish peroxidase (HRP) or sugar-lectin biospecific interaction between concanavalin A (ConA) and glucose oxidase (GOD) through LBL assembly. The developed bienzyme biosensor exhibited fast amperometric response for the determination of glucose. The linear response of the developed biosensor for the determination of glucose ranged from 2.0×10-6 to 1.7×10-4 mol L-1 with a detection limit of 2.5×10-7 mol L-1. The biosensor can be used to determine glucose in serum directly.2. A signal amplification probe was constructed based on functionalized CNTs labeled with HRP and HRP-secondary antibodies (HRP-Ab2).The enzyme was assembled onto CNTs using LBL assembly and sugar-lectin biospecific interaction. Greatly amplified sensitivity was achieved by loading a large number of enzymes througe LBL assembly.3. A sandwich-type amperometric immunosensor for carcinoembryonic antigen (CEA) was fabricated by using functionalized CNTs labeled with HRP and HRP-Ab2. The gold electrode was modified by monlayer cysteine on which carcinoembryonic antibody(Ab1) was immobilized. The developed sandwich-type amperometric immunosensor exhibited fast amperometric response for the determination of CEA. The response is linear in the range of 0.05 to 5 ng mL-1 and 5 to 200 ng mL-1, with a detection limit of 0.018 ng mL-1. The biosensor can be used in serum directly.

Related Dissertations

  1. Expression and Antibody Preparation of sglt1 from Cyprinus CarpioL.,S917.4
  2. The Effect and Underlying Mechanism of Resveratrol- mediated Cellular Calcium and Glucose Utilization in Rat Cortical Neurons,R285.5
  3. The Effects of Yizhi Jiannao Granule Concentration Fluid (YCF) on the Expression of IR、GLUT4 mRNA in the Hippocampus of AD Rats,R285
  4. The Study That Reactive Oxygen Species Activates Mitochondrion Apoptosic Pathway in Cardiac Myocytes Apoptosis by High Glucose,R587.2
  5. The Role of Oxidative Stress and Endoplasmic Reticulum Stress on Apoptosis in Neonatal Rat Cadiocyte Induced by High Glucose,R587.2
  6. Changes and Mechanisms of Myocardial AMPK Phosphorylation and Glycogen Content in Rats Exposed to Hypobaric Hypoxia,R594.3
  7. The Mechanism and Action of Glucagon-like Peptide-1 on G6Pase of Gluconeogenesis of HepG2Cells,R587.1
  8. Clinical Observation on the Efficacy and Safety of Biphasic Insulin Aspart 30 for the Treatment of 234 Patients with Type 2 Diabetic,R587.1
  9. Effects of New Intravenous Infusions on Blood Glucose Variability in Elderly Type 2 Diabetic Patients,R587.1
  10. Effect of Blood Glucose Fluctuation on Renal Pathological Change and Collagen Ⅳ Expression of Diabetic Rats,R587.1
  11. Igh Permeability Glucose Solution Caused Athological and Physiological Changes in Dorsal Root Anglion in Rats,R614
  12. Clinical Study on Pulse Pressure Variation-directed Intraoperative Fluid Therapy,R614
  13. The Roles and Mechanism of HIF-2α in Pathogenesis of Ischemic/Hypoxic Acute Kidney Injury,R692
  14. Effect of MicroRNA302c on Epithelial-mesenchymal Transition in Peritoncal Mesothelial Cells,R692.5
  15. The Effect of Dexamethasone on Glucose Metablism of Pregnant Women for Fetal Lung Maturity,R714.2
  16. The Effects of Clozapine and Olanzapine on Glucose Transporter 4 Gene Expression in Skeletal Muscle in Rats,R749.3
  17. The Study of Preparation Electrochemical Properties and Surface Modification of Boron-doped Diamond Thin Film,TB383.2
  18. Research on MWCNT Modified Electrode Degrading Biochemical Treated Coking Wastewater,X703
  19. Development of Ru/C Catalysts for Hydrogenation of Glucose to Sorbitol,O643.36
  20. The Influence of Glucose Variability on Prognosis in Critically Ill Patients,R587.1
  21. Compare Different Indexes of Blood Glucose Fluctuation to Investigate the Practical Application in Blood Glucose Monitoring of Diabetes,R587.1

CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
© 2012 www.DissertationTopic.Net  Mobile