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Technologies for Lactosucrose Synthesized by β-fructofuranoside

Author: LiaoChunLong
Tutor: YinYuLong;RuanZheng
School: Nanchang University
Course: Agricultural Products Processing and Storage
Keywords: Lactosucrose Enzymatic Synthesis Decolorization Purification Detect
CLC: TS241
Type: Master's thesis
Year: 2010
Downloads: 106
Quote: 0
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Lactosucrose (O-β-D-galactopyranosyl-(1 → 2)-β-D-fructofuranoside) is a functional oligosaccharides, having a series of physiological functions, such as low calorie, difficult to digest, and promote bis bifidobacteria proliferation, inhibit pathogens, anti-caries effect, adjust bowel function, lowering blood pressure, lowering cholesterol, promote mineral absorption features, great development prospects. In China, Lactosucrose can be said that has just started its industrialization in the country has not been reported, due to the purification of the enzyme activity in the production and sugar restricted its development in the country. This topic intends to study microbial enzymatic synthesis process Lactosucrose conditions determine the best Lactosucrose synthesis program and the Lactosucrose decolorization, purification preliminary study, and provide technical parameters for future industrial production. This article's main findings and conclusions are summarized as follows: 1. Determine the optimum conditions of the crude enzyme solution synthesis of β-fructofuranosidase Lactosucrose. : Sucrose and lactose as substrate, the crude enzyme solution synthesis of β-fructofuranosidase Lactosucrose enzymatic synthesis process by univariate and Box-Behnken experimental response surface analysis, enzymatic synthesis of low poly lactulose optimum process parameters. Results: The optimum conditions are as follows: The reaction time is 22h45min pH value of 7.0, the reaction temperature was 35 ° C, substrate concentration of 20%, the volume of the substrate with the enzyme ratio of 1:1 (enzyme activity of 250U/mL) Lactosucrose content of 22.70%. Conclusion: Box-Behnken combined response surface optimization fructopyranoside Enzymatic Synthesis Lactosucrose process model is reliable and feasible method. 2. Determine Lactosucrose activated carbon bleaching process. Methods: neural network and genetic algorithm combination decoloration rate and recovery rate of decolorization process optimization. Uniform design data to create a neural network model, the input layer of activated carbon dosage, bleaching time and the bleaching temperature output layer decolorization rate and recovery rate, the use of genetic algorithm for multi-objective optimization of the network, to get the Pareto optimal solution. Results: Pareto optimal solution: the carbon dosage 2.1894-2.1897% the decolorization time to 64.05-64.06min bleaching temperature 74.22-78.90. Actual measurement value of the validation test decolorization rate of 94.85%, Lactosucrose recovery was 97.23%, the relative error of the actual and predicted values ??of 1.51% and 0.12%, respectively. Conclusions: The neural network combined with the genetic algorithm optimize Lactosucrose decolorization process, model reliable and feasible method to achieve more satisfactory bleaching effect. 3. Preliminary purification of the enzymatic synthesis Lactosucrose mixed sugar. Methods: lactose crystallization and ion exchange method Lactosucrose preliminary purification conducted a preliminary exploration on the part of the process conditions and the ion exchange method lactose crystallization conditions. Results: The optimum conditions are as follows: the lactosucrose mixed solution was concentrated to 25%, addition of lactose seed crystals are placed in a 4 ° C refrigerator for 2 days, removed, 4500r/min centrifugal 20min, the supernatant; then through the ion exchange The purified, the purification conditions for the injection volume 5mL, column temperature was room temperature, eluent flow rate was 2mL/min the horizontal selection of the column length is 5m. Were purified, mixed sugar content of Lactosucrose from 18.26% to 41.62%. Conclusion: Lactosucrose mixed sugar preliminarily purified lactose crystallization and ion exchange method, the content is increased to 41.62% from 18.26%. 4 for SME Lactosucrose industrialized production process in the real-time detection method. Methods: The combination of the thin layer separation and the phenol-sulfuric acid wears, n-butanol: glacial acetic acid: water = 8:3:1, color developing agent: Aniline - diphenylamine - phosphoric, strip dotted sample, the point sample volume 5μl. Results: The thin-layer chromatography may be separated from the respective components of the reaction liquid monosaccharide lactosucrose to three sugar, and by thin layer chromatography proved for the first time the presence of fructose in the enzymatic synthesis Lactosucrose sample by phenol sulfuric acid method analysis Lactosucrose linear range 10.8-39.6mg/mL (R2 = 0.9939), precision (RSD) of 1.51%, the average recovery rate was 97.28%, RSD 3.07%. Conclusion: The content of the food or soybean oligosaccharide, raffinose was detected by thin layer chromatography - the phenol-sulfuric acid method to control the measurement results with the HPLC method, and the relative error is 3.22%, the industrialization of the production process can be used for detection.

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