Dissertation > Excellent graduate degree dissertation topics show

Study on Purification of Porcine Hemoglobin by Ultrafiltration Membrane Separation

Author: LiuFeng
Tutor: WangXiang
School: Chongqing University
Course: Biomedical Engineering
Keywords: Ultrafiltration Pig hemoglobin Purification Blood substitutes
CLC: TQ464.56
Type: Master's thesis
Year: 2010
Downloads: 202
Quote: 1
Read: Download Dissertation

Abstract


Worldwide blood shortage of resources, coupled with emergency surgery, the occurrence of the earthquake, the growing importance of the status of blood substitutes. Hemoglobin blood substitutes as the main raw material, and its activity is closely related to the safety and purity of blood substitutes. The ideal hemoglobin raw material should have a high purity and concentration and is close to the natural activity of the hemoglobin in red blood. The current research results have been reported using different methods, respectively purified hemoglobin solution, but by different degrees of damage to the activity of the purified hemoglobin. Ultrafiltration is a raw active injury smaller means of membrane separation techniques. Based on this characteristic, the experimental introduction of ultrafiltration technology to hemoglobin purification to high purity purified, and close to the natural red blood cells, hemoglobin activity hemoglobin solution. About this study: Objective: To establish and optimize hemoglobin purified ultrafiltration conditions; purified Institute of artificial red blood cells need a high purity and concentration and has natural red blood oxygen carrying capacity of pig hemoglobin solution. Methods: first by hypotonic extracted from pig's blood erythrocyte hemoglobin solution, then purified pig hemoglobin using ultrafiltration membrane separation. According to the relationship between the membrane flux ultrafiltration operating pressure and feed temperature analysis to establish optimal ultrafiltration purification conditions, and ultrafiltration under conditions optimized purification pig hemoglobin. And electrophoresis, UV - visible spectrophotometry of purified hemoglobin identified and analyzed by SDS-polyacrylamide gel (SDS-PAGE). In addition, the application of oxygen analyzer hemoglobin solution after ultrafiltration determination and P50 determination of the oxygen-carrying capacity. Results: In the process of ultrafiltration of hemoglobin, the membrane flux with the operating pressure is substantially linear relationship between the increase optimized ultrafiltration conditions were as follows: operating pressure 0.05Mpa, the feed temperature was 12 ° C; Under this condition, the separation and purification of porcine hemoglobin , purity over 99%, the concentration before purification improved about 20 times. Oxygen analyzer, after purification by ultrafiltration pig hemoglobin P50 20.42, close to the natural red blood cell S-type oxygen carrying curve. Conclusion: This topic using ultrafiltration purified porcine hemoglobin, not only to achieve a certain amount of concentration and purity standards, and through the detection of the oxygen-carrying capacity, proved less damage to the activity of the hemoglobin. A further study on this topic ultrafiltration purified hemoglobin our laboratory blood substitutes to provide a better feedstock. Ultrafiltration is a membrane separation technology common, has a non-polluting, under mild conditions, less protein denaturation and recovery high. Therefore, this issue is the method the ultrafiltration purified hemoglobin in the future is expected to be developed industrialized.

Related Dissertations

  1. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  2. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  3. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  4. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  5. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  6. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  7. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  8. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  9. Under microaerobic conditions closed calcium carbide furnace exhaust COS gas adsorption purification,X781
  10. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  11. Experiment Study of the Biogas Purification Process by Zeolite Filling Washing,TQ221.11
  12. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  13. Extraction, Purification and Antifungal Activity of Anthocyanins in Drop Fruits of Chinese Baberry,TQ914.1
  14. Preliminary Research on Characterization of Thermophilic Protease Pasey and Helicase Htc16,Q814
  15. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  16. Purification of Human Respiratory Syncytial Virus Fusion Protein with Immunomagnetic Microsphere Technology,R373
  17. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  18. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  19. Screening of Bacterial Strains with High α-Galactosidase Activity and Its Gene Cloning, Expression, Purification and Characterization,TQ925
  20. Study on Extraction, Isolation and Antioxidant Activity of Lignans of Sesame Cake,TS229
  21. Lipase-catalyzed Transesterification of Lard to Produce L-ascorbyl Fatty Acid Esters,TS221

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > Drug production of biological products > Organ preparations > Blood products
© 2012 www.DissertationTopic.Net  Mobile