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Nano-micelles of Amphiphilic Polysaccharide Derivatives for Encapsulation of Photosensitizers

Author: WangDan
Tutor: YangLiQun
School: Sun Yat-sen University
Course: Polymer Chemistry and Physics
Keywords: Amphiphilic polysaccharide derivative Loaded micelles Photosensitizer Fluorescence Quenching Fluorescence quantum yield
CLC: TQ460.1
Type: Master's thesis
Year: 2010
Downloads: 135
Quote: 0
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Abstract


Polysaccharides (eg, chitosan, sodium alginate, etc.) exists widely in nature, non-toxic, and has a very good biocompatibility and biodegradability, is the ideal drug carrier raw materials. Amphiphilic polysaccharide derivative coupling some of the good biocompatibility of the hydrophobic group, a hydrophilic polysaccharide chain can be synthesized in the aqueous solution, they can be self-aggregation forming nano-micelles as drug carriers. Photodynamic therapy is a use of photosensitizers generate singlet oxygen under visible light irradiation to kill tumor cells, which play a new method of treating tumor effect. Treatment efficiency of the tumor through photodynamic action to improve the photosensitizer, the topics, the work load photosensitive amphiphilic polysaccharide derivatives Nanomicelles papers and major findings include: (1) to chitosan as raw material, and alginic acid synthesized two amphiphilic polysaccharide derivatives, deoxycholic acid group-containing chitosan derivatives (Chit-DC) and sodium alginate derivatives containing cholesterol group (Alg-Chol ). Infrared Spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (1H NMR) analysis of the results showed that the hydrophobic group in the chemical structure of the two amphiphilic polysaccharide derivative through an ester bond between the main chain coupled with the hydrophilic polysaccharide . Chit-DC and Alg-Chol, Micellization Behavior in PBS (pH6.2) solution by pyrene fluorescent probes Law Research results show that they can form a stable micelles were measured critical aggregation concentration ( CAC) of 0.22 and 0.19 mg / mL. (2) in PBS (pH6.2) solution, through supramolecular assemblies were prepared both load Photofrin (carboxy porphyrin derivatives) and TAPP (four (4 - aminophenyl) porphyrin) photosensitizer kinds of drug-loaded micelles, they were named Chit-DC-Photofrin, Chit-DC-TAPP, Alg-Chol-Photofrin and Alg-Chol-TAPP. UV-visible spectrometry proved to the photosensitizer molecules have been dispersed into micelles. Dynamic light scattering results Chit-DC-Photofrin and Chit-DC-TAPP micelle hydrodynamic diameter of approximately 400-500 nm, Alg-Chol-Photofrin and Alg-Chol-TAPP hydrodynamic diameter of the micelles is about 100 -200 nm. Observed through TEM the Chit-DC-Photofrin and Alg-Chol-TAPP micelle showed a spherical shape, their diameters were about 300 nm and 100-200 nm. Zeta potential analysis of the results showed that the the photosensitizer molecules interested uncharged micellar nanoparticles surface charge properties have a great impact. (3) by the steady-state fluorescence spectroscopy the load photosensitizer amphiphilic polysaccharide derivatives Nanomicelles photodynamic performance, the results showed that Photofrin amphiphilic polysaccharide derivatives Nanomicelles load, the fluorescence quantum yield can be significantly increased, which Alg-Chol-Photofrin micellar system the Chit-DC-Photofrin micellar system has a higher fluorescence quantum yield. TAPP amphiphilic polysaccharide derivatives Nanomicelles load, the fluorescence quantum yield has also been significantly improved. Photofrin in PBS (pH = 6.2) solution fluorescence quenching concentration of about 8 × 10-6g/mL TAPP fluorescence quenching in THF solution concentration of about 1 x 10-5 g / mL. (4) cell experimental results show that the the Alg-Chol-Photofrin micelles by photodynamic effects on human pancreatic cancer cell line Panc-1 to the killing effect of its mechanism of action and its induction of cell necrosis and apoptosis.

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