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Research on Extraction Technics and the Function of Antioxidation and Fat Metabolization Regulation for Ralls Apple Polyphenols

Author: ZhouLiPing
Tutor: WangZhenYu
School: Northeast Forestry University
Course: Forest plant resources ,
Keywords: Apple Polyphenols Extract Antioxidant Fat metabolism
CLC: S661.1
Type: Master's thesis
Year: 2010
Downloads: 188
Quote: 7
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Abstract

Apple Polyphenols as natural antioxidants can inhibit or block the oxidation process, balancing free radicals, so the researchers caused widespread concern. In this paper, Ralls Apple as the research object, using ultrasonic assisted grinding beads were determined Ralls apple polyphenol antioxidants and optimal extraction process, using macroporous resin adsorption separation technology to prepare the apple polyphenols, its antioxidant and fat Metabolic regulation function were studied, aimed at further development and utilization of apple polyphenols provide a reference. Specific findings are as follows: (a) single-factor results show bead extraction method can effectively extract Ralls apple polyphenols. (2) Response surface methodology to determine the total phenolic Ralls Apple optimum extraction process as follows: ultrasonic time of 22.62 min, ultrasonic temperature 50.38 ℃, ultrasonic power 243.85 W, load capacity grinding beads (beads: material g / g) 1.25:1; Ralls Apple flavonoids extraction technology: ultrasonic time 22.75 min, ultrasonic temperature 51.52 ℃, ultrasonic power 229.35 W, load capacity grinding beads (beads: material g / g) 1.21:1; Ralls apple procyanidins extraction technology: Ultrasound time of 21.17 min, ultrasonic temperature 51.09 ℃, ultrasonic power 249.83 W, grinding beads load capacity (beads: material g / g) 1.22:1; polyphenolic antioxidants apple optimum extraction process: ultrasonic time 23.75 min, ultrasound Temperature: 50.81 ℃, ultrasonic power 244.8W, load capacity grinding beads (beads: material g / g) 1.21:1. (3) through static adsorption, analytical experiments to determine the optimal separation Ralls apple polyphenol stationary phase X-5 macroporous resin, Ralls apple polyphenol obtained up to 70% purity. (4) for Ralls apple polyphenol antioxidant activity in vitro experiments showed that apple polyphenols have strong total antioxidant capacity and restore capabilities, and can effectively scavenge hydroxyl radicals, superoxide radicals, DPPH radicals and Hydrogen peroxide radicals. (5) used to establish high-fat animal model to study the apple polyphenols on the regulation of fat metabolism in mice, the results show that apple polyphenol extract and purified extracts of apple polyphenols can significantly reduce blood triglyceride mice , total cholesterol, low-density lipoprotein (LDL-C) and MDA and atherosclerosis index (AI), right mouse serum HDL-C significantly increased effect, can effectively improve the superoxide mice dismutase, glutathione peroxidase, lipoprotein lipase and hepatic lipase activity, which has lipid-lowering, regulate fat metabolism functions. (6) for Ralls apple and other varieties of apples comparison shows that the composition and functions, Ralls Apple's total phenolic content than other six varieties of apples, and the total content of flavonoids and proanthocyanidins were inferior bells and Huang Taiping apple fruit, in second place. Ralls Apple's total antioxidant capacity after Huang Taiping apple, ranked second; ability to scavenge hydroxyl radicals behind 77-6, also in second place.

Research on Extraction Technics and the Function of Antioxidation and Fat Metabolization Regulation for Ralls Apple Polyphenols

Abstract

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