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Objective: This article by observing different cardiac function in heart fatty acid binding protein (heart-type fatty acid-binding protein, H-FABP) and brain natriuretic titanium (B-type natriuretic peptide, BNP) content changes discussed both in The clinical value of chronic heart failure (chronic heart failure, CHF) and TCM heart yang relationship. Methods: specific pathogen-free (SPF) 40 the ordinary male Wistar rats, body weight 200 ± 20g, randomly divided into four groups: saline control group of 10, the model group of heart failure two weeks, four weeks, and 8 weeks 10 each. 1 CHF model: 30 healthy male Wistar rats, body weight 200 ± 20g, were randomly divided into three groups, with 1% sodium pentobarbital 20 ~ 25mg/kg intraperitoneal injection of anesthesia, according to 85mg/kg subcutaneous injection of isoproterenol (isoprotereno, ISO), 24h after repeated once. Two weeks after treatment, 4 weeks, 8 weeks, cardiac function testing. Normal control group: Take 10 healthy male Wistar rats, weighing 200 ± 20g, 20 ~ 25mg/kg intraperitoneal injection of anesthesia with 1% sodium pentobarbital, injected with normal saline 0.25ml after 24h Repeat 1 times. 3 model identified: (1) cardiac function testing: in the second weekend four weekends and eighth weekend of the model group, cardiac function testing: measurement of left ventricular systolic pressure (Left ventricular systolic pressure, LVSP) via the carotid artery left ventricular end-diastolic pressure (Left ventricular end-diastolic pressure, LVEDP) and left ventricular pressure increase and decrease speed (Left ventricular developed pressure ± LVdp / dt) (2) HFABP and the BNP assay: enzyme-linked immunosorbent assay determination the plasma HFABP and BNP content. (3) Pathology slice detection: paraffin-embedded sections of the left ventricle, HE staining. (4) left ventricular mass index (left ventricular weight mass index, VWI) (Vw / Bw) and lung, liver mass index was measured. (5) Data analysis: SPSS13.0 software analysis system for processing the data obtained. All indicators are mean ± standard deviation (x ± s), said the groups were compared using one-way ANOVA, P lt; 0.05, statistically significant results. The results: 1. Rats generally and mortality: CHF model group rats 2 weeks group 2 died, two died 4 weeks, 8 weeks, two died, the mortality rate was 20%. The cause of death may be malignant arrhythmia or congestive heart failure, and NS group with no deaths, the group between the rates of P lt; 0.05. Symptoms of CHF model group and the NS group, significant differences in CHF rats compared with NS group mental fatigue, chills, fur gloss, coat the yellowish port cyanosis, and breathing asthma, reduced food intake and activity, oliguria. Slow weight gain, paw edema, poor response to crawl, in line with the traditional Chinese medicine heart Yang Qi type. 2. Changes in rat cardiac pathology: heart dim specimens observed: ISO group of the surface of the heart, dark purple color; the NS group heart surface smooth, ruddy color, non-inflammatory lesions. HE staining showed that the CHF group myocardial tissue damage: (1) myocardial fibers disorganized, hypertrophy, degeneration and necrosis, part myofibrillar dissolved or broken. (2) myocardial interstitial congestion, edema, inflammatory cell infiltration, proliferation of connective tissue; (3) into fibroblast proliferation and myocardial interstitial fibrosis. As ventricular remodeling, the lesion severity. NS Group myocardial fibers arranged in neat rows, clear nucleus, the cell gap is normal, and the destruction of myocardial fibers. 3 rat blood flow dynamics determination: CHF2 weeks group comparison between the NS group, P gt; 0.05, no statistical difference between the two; the CHF model 4 weeks and 8 weeks compared between NS group, CHF model group between any two, LVSP, ± LVdp / dtmax were significantly decreased (P lt; 0.01), LVEDP increased (P lt; 0.01), compared to twenty-two statistically significant. Left ventricle / body weight, lung / body weight, liver / body weight ratio: compared with the NS group, CHF model group mass index was significantly increased, which model of CHF 4 weeks and 8 weeks compared with NS group (P lt ; 0.01), a statistically significant difference; model group, model of CHF 2 weeks compared with the NS group (P gt; 0.05), no statistically significant difference. Rat plasma BNP levels: BNP with the severity of heart function gradually increased between the NS group and CHF group two weeks, pairwise comparisons between CHF 2 weeks and 4 weeks, respectively, P lt ; 0.05, NS group and CHF group four weeks, eight weeks between the pairwise comparison, P lt; 0.01, among the statistical difference. 6. Rat plasma HFABP content: HFABP with the severity of heart function and gradually increased to CHF group and NS group, pairwise comparisons between CHF model group, HFABP were significantly higher (P lt; 0.01), statistical comparison between groups learning differences. Conclusion: 1.BNP plasma concentrations cardiac function failure (CHF) aggravated gradually increased content of plasma BNP and cardiac function (LVdp / dtmax) were negatively correlated, r = -0.855 2.HFABP plasma concentrations of heart failure (CHF) heavier gradually increase, the plasma HFABP content and cardiac function (LVdp / dtmax) was a negative correlation, r = -0.893. 3 from the normal group, the heart failure group, group model of CHF 2 weeks to 8 weeks, plasma HFABP level BNP The level was positively correlated, r = 0.914. 4. As can be seen from the model group, the degree of myocardial fibrosis aggravated over time, suggesting myocardial reconstructed in the CHF degree gradually worsened.
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