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Icariin Protects Against Brain Injury and Its Molecular Mechanism in Experimental Stroke

Author: ZhuHaiRong
Tutor: XuYun
School: Nanjing University of Traditional Chinese Medicine
Course: Chinese medicine
Keywords: Icariin Ischemic brain injury PGC-1α SIRT1
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 116
Quote: 0
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Abstract


Icariin(ICA), the major active component of traditional Chinese herb "Yinyanghuo", has various pharmacological effects.The main pharmacological activity of ICA is to improve the cardiovascular system function, enhance the immune and regulate endocrine, as well as anti-tumor, anti-virus effect. In this study we investigate the neuroprotective effect of ICA on ischemic brain injury and the possible mechanisms both in vivo and in vitro.Specially, the unique target of ICA is under our search. This research also provides the experimental basis for further study pharmacological effects of ICA on ischemic brain injury.Objective:To investigate neuroprotection of ICA on ischemic brain injury and underlying neuroprotection mechanisms.Methods:1.The mice model of ischemic brain injury was made by middle cerebral artery occlusion. The behavioral test and brain edema were detected and infarct volume was measured by TTC.The expression of PGC-la and Sirtl in ischemic cortex were detected by Western blot.2. Primary cerebral cortical neurons were cultivated. Then they were subjected to OGD.The neuronal viability was determined by MTT assay.The flow cytometry was used to assess the cell death.The expression of PGC-la mRNA was identified by real time PCR in order to detect the efficiency of PGC-la siRNA. Western blot was used to evaluate the expression levels of PGC-la and Sirtl protein in vitro.Results:1.Compared to the vehicle, ICA group shows decreased neurological scores, reduced cerebral water content and infract volume;2.ICA enhances the protein expression of Sirtl and PGC-la;3.ICA can enhance the cell viability and reduce mortality of neuronal OGD model.The Sirtl inhibitorⅢor PGC-la siRNA can reverse the neuroprotective effect of ICA.4. ICA increases the protein expression of Sirtl and PGC-la. The enhance of PGC-la by ICA can be reversed by Sirtl inhibitorⅢ.Conclusions:The results suggest ICA has neuroprotection on ischemic brain injury. The mechanism on neuroprotection of ICA may upregulate PGC-1αmediated through Sirt1.

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