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Objective: fine of felting wool honeysuckle (Lonicera similis Hemsl) the plant fine felting wool honeysuckle Lonicera dried buds or open flowers (Xi said, a long Diaozi Yinhua). Antibacterial anti-inflammatory, detoxify, cool scattered wind-heat effect, mainly used in the treatment of carbuncles boils the Houbi, erysipelas Noxious Heat dysentery, Fengreganmao, warm the incidence of other diseases. Its main active ingredient is chlorogenic acid, chlorogenic acid, total flavonoids. Mainstream varieties of the fine carpet hair honeysuckle honeysuckle in Sichuan and Chongqing. The characteristics of the subject will Sichuan, Chongqing, producing fine felting wool honeysuckle pharmacognosy study its TLC, and the determination of the content of the active ingredient, its fingerprint, to improve and perfect fine Sichuan and Chongqing production blanket Lonicera quality control methods. Method: full control of production quality standards for fine felting wool honeysuckle to Sichuan and Chongqing, the subject of the original plant identification, character identification, microscopic identification of Sichuan, Chongqing, producing fine felting wool honeysuckle pharmacognosy identification using thin-layer chromatography qualitative identification of chlorogenic acid, using UV spectrophotometric determination of total flavonoids and total chlorogenic acid content of chlorogenic acid content was determined by high performance liquid chromatography and high performance liquid chromatography of their fingerprint The map study provide a scientific basis for the development and utilization of Sichuan, Chongqing, producing fine felting wool honeysuckle. Results: The original plant is a perennial semi-evergreen lianas, young shoots, petioles and peduncle are carried out long coarse hair, pale yellowish-brown, pubescent and sparse glandular hairs, double flowers single born in the leaf axils or minority set twigs end racemes. The herbs have buds fine wooden club-like surface for the yellow-brown, red, brown or green, non-glandular hairs sparse, glandular hairs more feature. Pollen microscopic characteristics with two glandular hairs cells, thick-walled non-glandular hairs cells, thin-walled non glandular hairs single cell, pollen grains: spherical clusters of calcium oxalate with the 3 colporate angular fine tip. Chlorogenic acid; detected using UV spectrophotometric determination of total flavonoids and total chlorogenic acid, a good linear relationship in the range of 0.01 ~ 0.08 mg / mL, measured Chuan Yin-hua in total flavonoids (TLC R = 0.9998), the recovery rate was 97.71%, RSD was 1.48%. HPLC determination of chlorogenic acid, the 0.8-8μg within a good linear relationship (r = 0.9999), the average recovery rate was 98.34%, RSD 0.91%. Fingerprinting studies using high-performance liquid chromatography, and measured 11 Total peak herbs, a total peak parameters in line with the relevant provisions of the TCM injections fingerprint technology requirements. Conclusion: The TLC of chlorogenic acid composition containing fine felting wool honeysuckle; UV spectrophotometric determination of total flavonoids and total chlorogenic acid content, the method is simple, rapid, sensitive, good repeatability and recovery can be As fine felting wool honeysuckle total flavonoids and total chlorogenic acid content determination method. HPLC determination of chlorogenic acid content, the method is simple, fast, with good reproducibility and recovery, as Sichuan and Chongqing production method for the determination of the fine of felt wool honeysuckle in chlorogenic acid. Fingerprinting studies using high performance liquid chromatography method is accurate, reproducible, the fingerprint testing standards established, one of the important basis for quality evaluation as a fine carpet hair Lonicera species identification.
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