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Degradation of Dibenzothiophene by Some Newly Isolated Bacterial Strains

Author: SongJing
Tutor: KangJianXiong
School: Huazhong University of Science and Technology
Course: Ecology
Keywords: Biodesulfurization Dibenzothiophene Filter Appraisal Optimization Metabolic pathways
CLC: TE621
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
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Abstract


Dibenzothiophene (DBT) is the major heterocyclic organic sulfides in the petroleum the traditional desulfurization method , and it is difficult to be removed . So often in the study of biological desulfurization of DBT as a model compound . In this paper, a plant height of activity can specifically digest the DBT desulfurization bacteria 6-1. 16S rDNA gene sequence analysis showed that the 16S rDNA gene of strain 6-1 DBT as the sole sulfur source , isolated from petroleum-contaminated wastewater sequence length of 1469 bp, 99% of the 16S rDNA gene sequence of the gene sequence of Cronobacter turicensis consistency . Therefore , the preliminary identification of the strain was Cronobacter turicensis . The growth curve of the strain found by measuring the growth of strain 6-1 after about 12h into the logarithmic phase , logarithmic phase lasted about 50h . Since then its growth into a stable period stabilization period lasted about 40h , the strain cell concentration during essentially the same . Found by measuring the strain desulfurization curve , from 36h to 48h , the the DBT concentration from the initial 0.2 mmol / L degraded to 0.0275 mmol / L, DBT degradation rate of 86.75% . By five groups of single factor experiments and orthogonal experiments , the desulfurization bacteria 6-1 culture conditions were optimized to obtain the optimal culture conditions of strain 6-1 : initial pH 7.0 , fermentation temperature of 30 ° C ; the best carbon source was of 2g / L glycerol; optimum nitrogen source was of 2g / L ammonium chloride ; optimum initial DBT concentration of 0.2mmol / L . Bacterial metabolic pathway of DBT degradation study using gas chromatography mass spectrometry . The fermentation was subjected After acidification and extraction, respectively , in the retention time of 8.809min and 9.204min detected the intermediate metabolite of n - dodecane and 4,5 - dimethyl octane . Final metabolites CO the 2 H 2 O , the organic sulfur is oxidized to sulfate ions released into the aqueous phase . Thus by controlling the oxygen content or by metabolic engineering methods , inhibiting further oxidation of n-dodecane and 4,5 - dimethyl octane metabolic reactions , so as to achieve efficient desulfurization at the same time , retain the thermal value of the oil .

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CLC: > Industrial Technology > Oil and gas industry > Oil, natural gas processing industry > Petroleum refining > Basic theory
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