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Mulberry Endophytes gap or cell survival in the cells of various tissues and organs of healthy mulberry, and to establish harmony between the host Interactions between a class of microbes, some endophytes in the the mulberry interaction with the host can produce rich bioactive secondary metabolites. But there has not been generated by the active metabolite on mulberry endophytic fungi and related applications reported. Mulberry endogenous the common bean shell ball cinerea MOD-1 (Mulberry endophyte Macrophomina phaseolina MOD-1) production of oil in our laboratory isolated from healthy mulberry root screened a fungus (GenBank accession number EU250575) was determined bacteria body Oily amounted to 22.72%. Thesis MOD-1 strains, optimization of its the lipid production medium and fermentation condition of, and to explore the feasibility of starch factory wastewater culture media, fermentation production of microbial oil. The main contents are as follows: 1. Macrophomina phaseolina MOD-1 Best lipid production fermentation medium and the best shake flask fermentation conditions optimized. The single factor screening method to determine the best carbon source for fermentation medium formulation for soluble starch, the best nitrogen source was peptone best metal salt ions as manganese chloride; Uniform Design finalize the fermentation of the strains produced grease medium optimal formula as follows: Soluble starch 105 g / L, peptone 1.1 g / L, potassium dihydrogenphosphate 1.5 g / L, ammonium sulfate 0.3 g / L, magnesium sulfate 0.32 g / L, and manganese chloride 4.9 nmol / L. On this basis, the use of single-factor method of fermentation conditions optimized the best shake flask fermentation conditions were: initial pH 7.0, temperature 26 ℃, rotation speed 190 r / min, liquid volume in 100 mL. Cultured in the medium and fermentation condition of optimized 6 d, the amount of cell growth (dry mass) of up to 41.852 g / L, oil production reached 25.511 g / L. By gas chromatography - mass spectrometry determination of Macrophomina phaseolina MOD-1 oil fatty acids in the optimized medium and fermentation conditions. The former detected 9 fatty acid composition, which monounsaturated fatty acid content of 67.92%; the latter detected seven kinds of fatty acid composition, which monounsaturated fatty acid content of 74.32%. After optimization MOD-1 strains increased oil production, the composition is simple, easy to purify, and monounsaturated fatty acids were higher. 3. Starch factory wastewater fermentation substrate Macrophomina phaseolina MOD-1 fermentation production of microbial oil. Studies have shown that this strain can effectively use synthetic oils plus any nutrients corn starch factory wastewater, the amount of cell growth (dry mass) of 2.218 g / L, the mycelium in the production of oils and fats to 1.005 g / L, and fat content reached 45.33%, 30.68% COD removal efficiency. Adjusted by adding carbon source - soluble starch the starch wastewater medium N ratio, in order to achieve the purpose of improving the strains MOD-1 bacterial production of oils and fats. The best fermentation C / N of 50:1, strains MOD-1, the amount of cell growth (dry mass), fat yield, fat content and COD removal were 35.773 g / L, 19.532 g / L, 54.60% and 54.40%, respectively. Grease under the conditions of this fermentation were detected in seven kinds of fatty acid composition, which is mono-unsaturated fatty acid content of 70.823%, the study showed that starch factory wastewater fermentation production of microbial oil while reducing wastewater COD value in resource utilization.
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