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The Method for the Determination of β2-agonists Residues in Foodstuff of Animal Origin by HPLC-MS/MS

Author: GuoDeHua
Tutor: LuZhaoXin
School: Nanjing Agricultural College
Course: Food Engineering
Keywords: β2-agonists foodstuff of animal origin HPLC-MS/MS
CLC: TS207.3
Type: Master's thesis
Year: 2008
Downloads: 8
Quote: 0
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Abstract


The method for the determination ofβ2-agonists residues in liver and meat of pig by HPLC/MS-MS (High Performance Liquid Chromatography-Tandem Mass Spectrometry) has been developed.All of P 2-agonists in this project are salbutamol, terbutaline, cimaterol, cimbuterol, ractompamine, clenbuterol, bromobuterol, isoxsuprine, mabuterol, mapenterol, bromclenbuterol.The sample was extracted in acetate buffer of pH 5.2 and extraction was hydrolyzed byβ-Glucuronidase/aryl sulfatase. Then the protein was precipitated with HCIO4. The supernatant was passed through a SCX cation exchange column (MCX,3cc,60mg), Clenbuterol-D9 & Salbutamol-D3was used as an internal standard, and then elevenβ2-agonists can be determined at one time.The experimental results from spiked liver sample indicated that the average recoveries of external ranged from 31.26%-79.88% and the recoveries ranged from 89.4%-110.5% and the RSD were 1.1%-2.8%. The limits of detection (LODs) were 0.5ng/g.

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CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > General issues > Food standards and testing > Food analysis and testing
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