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Studies on Microsatellite Marker and Morphological Contrastive Analysis of Wild and Cultivated Pteria Penguin (r Ding)

Author: WangYongLi
Tutor: WangMeiFang
School: Guangdong Ocean University
Course: Aquaculture
Keywords: Pteria penguin Microsatellite markers Morphological analysis
CLC: S917.4
Type: Master's thesis
Year: 2010
Downloads: 53
Quote: 0
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Abstract


In this experiment, the penguin pearl oyster (Pteria penguin Roding) for the study, for the first time from the level of the DNA molecule and morphological level Pteria penguin two groups were compared . From 85 to its close relative martensii primer Pteria penguin 26 pairs of primers amplified specific bands . Using 26 pairs of primers for amplification and population genetics analysis of wild and farmed populations . 26 sites , the average single -bit fixed point coefficient (Fst) value of 0.0434 ; average polymorphism information content (PIC) value of 0.5638 ; wild and cultured populations of the average number of alleles , respectively, 3.8 and 3.4 ; average observed heterozygosity (Ho) were 0.6286 and 0.4685 ; average expected heterozygosity (He) were 0.6610 and 0.5539 ; Shannon's diversity index (I *) were 1.1535 and 0.9086 ; the the inbred line number (Fis) were 0.0922 and 0.0931 , results that cultured population genetic diversity and the degree of inbreeding was higher than the wild population , but still showed good genetic diversity . Starting to lay the future Pteria penguin microsatellite primer screening primers . Wild and farmed penguin pearl shell of shell length , vertical shell , the ventral edge of the shell high as the independent variable shell width traits as the dependent variable related coefficient path coefficients , the coefficient of determination and the related index such as , for analysis , the results show that : the wild shell width and three morphological traits in the breeding population , respectively : shell length gt ; the vertical shell height gt ; ventral edge of the shell , the the vertical shell height gt ; ventral edge of shell height gt ; shell length, and have reached a very significant (p lt; 0.01) or a significant level ( p LT ; 0.05 ) ; final quantization parameter X1 in the two groups ( SL / ST ) , X2 ( the SW / ST ) , X3 (SH / ST ) of the mean, variance analysis, have to shell length / ventral edge of shell height can be used as indicators to distinguish between the two groups . This study Pteria penguin protection and utilization to provide a theoretical basis for the determination of ideal indicators .

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
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