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miR-206 Inhibits CDC42 Protein Expression, Invasion and Migration of MDA-MB-231 Cells

Author: LiuHao
Tutor: CaoYouDe
School: Chongqing Medical University
Course: Pathology and Pathophysiology
Keywords: Breast tumor Attack Shift miR-206 Cdc42
CLC: R737.9
Type: Master's thesis
Year: 2010
Downloads: 56
Quote: 0
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Abstract


Background and Purpose : microRNA is a length of only 19 to 30 nucleotides in non-coding RNA , mRNA degradation or inhibit translation process can be combined with the mRNA 3 ' non- coding region , which play the role of a negative regulator . The study found , the microRNAs occurrence of many diseases , the development process has a regulatory role , including the tumor . This study was to investigate the regulation of miR-206 , MDA-MB-231 breast cancer cells Cdc42 protein expression and cytoskeleton and cell invasion , migration ability . Methods: LipofectamineTM 2000 transfected miR-206 into the MDA-MB-231 cells 48 h after treatment , Western blot detection of breast cancer cells after transfection Cdc4 protein expression of MMP -2 and MMP-9 . Immunofluorescence microscopy to observe changes in cell morphology and cell filopodia and counted the number of cells pseudopodia ; further invasion and migration assay cell invasion and migration capability changes before and after transfection . Results : Western blot detection of Cdc42 , MMP -2 and MMP-9 expression after transfection , striped gray value compared with the negative control group were significantly decreased ( P lt; 0.01 ) ; immunofluorescence counts each cell The average number of filopodia , blank control group (14.99 ± 5.53), epidermal growth factor (EGF) group (23.59 ± 3.92), miR-206 transfection group (9.45 ± 3.59 ) , miR -206 EGF group (11.77 ± 2.85). MiR -206 transfected group or miR-206 EGF group cell filopodia significantly reduced compared with the control group and the EGF group (P lt; 0.05). Invasion and experimental results show that the number of cells in the small chamber under the membrane blank control group (311.7 ± 23.5), miR-206 transfection group ( 65.0 ± 13.9 ) , the difference was statistically significant ( P lt; 0.05 ) . The migration experiment results show that the number of cells in the small chamber under the membrane blank control group (793.0 ± 76.3), miR-206 transfection group (415.3 ± 20.0), the difference was statistically significant ( P lt; 0.05 ) . Conclusions : miR-206 can inhibit the formation of MDA-MB-231 cells Cdc42 expression and cell filamentous pseudopodia , and can inhibit the invasion and migration of cells .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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