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Objective northeastern Sichuan Acinetobacter baumannii on β-lactam antibiotics and to analyze the reasons for their produce drug for clinical treatment of laboratory evidence; establish a rapid and efficient detection of Acinetobacter baumannii β-lactamase gene approach and to explore Acinetobacter baumannii β-lactamase gene and the presence of multiple drug resistance relationship; detection of carbapenem-resistant Acinetobacter baumannii OXA gene distribution and OXA gene regulatory sequences and expression. Methods agar dilution assay non-repetitive clinical isolates of Acinetobacter baumannii 90 for 20 kinds of commonly used β-lactam antimicrobial minimum inhibitory concentration. Multiplex PCR 90 Acinetobacter baumannii common five kinds of β-lactamase gene: PER-1, AmpC, TEM-1, SHV-5, CTX-M2; conventional PCR detected 21 carbapenem-resistant ene Acinetobacter baumannii in four OXA gene (OXA-23, OXA-24, OXA-51, OXA-58), Ⅰ integron gene (int Ⅰ) and insertion sequence gene (ISAba1, ISAba4). Results 90 Acinetobacter baumannii cefazolin, aztreonam, cephalothin, cefoxitin, cloxacillin, ampicillin, cefoperazone resistance rate of 83.3-94.4%. 64.4% of the sample is detected Acinetobacter baumannii 1 to 4 kinds of β-lactamase gene, wherein the PER-1 50.0%, AmpC 45.8%, TEM-1 35.1%, SHV-5 22.4%, CTX-M2 6.8% . β-lactamase gene positive strains of multi-drug resistant strains were higher than negative. In the 21 carbapenem-resistant Acinetobacter baumannii has eight Acinetobacter baumannii OXA-23-positive, four OXA-51-positive, which has two OXA-23, OXA-51 were positive, there is a strains of int Ⅰ gene-positive, the rest were negative. 10 OXA gene-positive bacteria in eight subjects ISAba1 positive, and that eight of OXA-23 were positive, ISAba4 were negative. Conclusions 1. Northeast Sichuan Acinetobacter baumannii to commonly used β-lactam antimicrobial agents widely. 2.β-lactamase gene in Acinetobacter baumannii in a high detection rate and a close relationship with its multi-drug resistance. 3 constructed multiplex PCR system can simultaneously amplify multiple β-lactamase gene for resistance genes in Acinetobacter baumannii study provides a fast and effective means of detection. 4 Acinetobacter baumannii carbapenem antibiotic resistance is one of the main OXA gene presence and expression; ISAba1 and OXA-23 genes are closely linked and may be involved in OXA-23 gene expression regulation.
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