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Carbapenem -resistant Acinetobacter baumannii related and Homology Analysis of resistance genes

Author: WangLiJuan
Tutor: ZhangChaoXia
School: Xinjiang Medical University
Course: Clinical Laboratory Science
Keywords: Acinetobacter baumannii Resistance Random amplified polymorphic DNA Carbapenemase
CLC: R440
Type: Master's thesis
Year: 2011
Downloads: 79
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Abstract


Objective: To investigate the First Affiliated Hospital of Xinjiang Medical University clinical isolates resistant to imipenem resistant Acinetobacter baumannii , homology and associated resistance genes . Methods: 42 pairs of imipenem -resistant Acinetobacter baumannii . Kirby-Bauer disk diffusion method imipenem -resistant strains 18 kinds of antimicrobial susceptibility , using PCR amplification carbapenemase genes include OXA-23, OXA-24, OXA-58, IMP, VIM, ultra- broad-spectrum β- lactamase gene , including TEM, PER, SHV and aminoglycoside enzymes including aac (3) - Ⅰ, aac (3) - Ⅱ, aac (6 ') - Ⅰ ad, aac (6') - Ⅰ b, aac (6 ') - Ⅱ, ant (3 \Results: 42 pairs of imipenem -resistant Acinetobacter baumannii resistant to amikacin lowest rate ( 28.5% ) , levofloxacin , gentamicin , ceftazidime resistance rates were 53.6% , cefoperazone / sulbactam resistance rate was 39.3% , and the remaining antimicrobial drug resistance rate greater than 80.0%; RAPD typing found 42 pairs of imipenem -resistant Acinetobacter baumannii are six types , of which A ( 8 ), D -type ( 20 ), F -type ( 6 ) 3 -type based. 42 pairs of imipenem -resistant Acinetobacter baumannii has 33 carries OXA-23 group of genes , aac (3) - Ⅰ 21株, aac (3) - Ⅱ 1株, aac (6 ') - Ⅰ b15 strains ant (3 \Conclusion: imipenem-resistant Acinetobacter baumannii resistance phenomenon is very serious , should be based on susceptibility results of clinical rational use of antibiotics , cloning is disseminated imipenem -resistant Acinetobacter baumannii main propagation way , OXA-23 -type is the most important carbapenemase , TEM, PER is the main β- lactamase , aac (3) - Ⅰ, aac (3) - Ⅱ, a, ac (6 ') - Ⅰ b, ant (3 \

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