|
Objective: In this paper, through modern separation and purification technology, analysis identification technology and multi-level activity detection technology on ginseng research less but the activity of a variety of biological macromolecules - proteins and peptides biological characteristics, process optimization ginseng concocted, The clinical material basis for the development of innovative drugs, health food products reveal ginseng provide a scientific basis and technical support. Methods: PAGE electrophoresis gel enzyme activity staining method, five kinds of garden ginseng (fresh ginseng, raw white ginseng), wild ginseng and forest Senate oxidoreductase, hydrolase, transferase, lyase, isomerase enzymes in a total of 22 kinds of enzyme activity. Select garden ginseng, wild ginseng Ginseng active enzymes were higher POD, CAT, SOD, MDH (oxidoreductases) and EST, ACP, AMY (hydrolases) different origin ( 10 origin), different parts (reed head, taproot, fibrous roots) and different years (4 years, 5 years) the garden ginseng (fresh ginseng), the enzyme activity was measured. Results: four kinds of parameters, a total of 13 enzymes with a clear enzymatic activity bands, of which, 11 kinds of fresh ginseng, eight kinds of dried white ginseng, wild ginseng, seven kinds of forest Senate. Peroxidase (POD), hydrogen peroxide enzyme (CAT), malate dehydrogenase (MDH), superoxide dismutase (SOD), esterase (EST), acid phosphatase (ACP), amylase ( AMY) 7 enzymes only fresh ginseng all enzymes, phosphoglucose mutase (PGM) and four parameters total of lactate dehydrogenase (LDH), cytochrome oxidase enzyme (CYT), aspartate transaminase (GOT) glucose phosphate isomerase (GPI) only wild ginseng all phosphate dehydrogenase (PGD) for a living white ginseng and fresh ginseng total. Conclusion: The four parameters activity differences, suggesting that ginseng, there is a certain quality differences due to the different cultivation methods, processing methods, which play a reference role for the ginseng species selection and clinical medicinal. Have a large difference of different origin and different parts of the ginseng enzyme; different years of ginseng variety of enzyme activity, although some differences, but not very different. Enzyme activity differences between the different origin of the ginseng, ginseng cultivation areas, there are some differences in the quality ginseng standardized planting some scientific basis; difference of ginseng in different parts of the enzyme, various enzymes participate in the distribution of the body different description the reed head taproot and fibrous root can not be ignored, which laid a theoretical basis for the overall ginseng medicine and clinical screening; 4-year and 5-year-old ginseng enzyme activity is not very different, description ginseng harvest life 4 years and 5 years can be.
|