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Objective: the pig stomach Wood (C. horridum Bl.) Rubiaceae (Rubiaceae) fish bone wood genus (Canthium Lam.) Plants . Pork bellies wood often as civilian medicinal use , the roots diuresis , swelling , pain, for jaundice , swelling , bruises ; leaves can be heat , anti-inflammatory , lungs and for sore , trauma, infections , tuberculosis . Therefore, the active ingredient of the Pork bellies wood extraction , separation , purification, characterization and chemical research , confirm pig stomach wood chemical composition , and to identify the active ingredients and structure , looking for compounds with pharmacological activity , Pork bellies wood development and utilization to provide a theoretical basis . Methods: tripe wooden stem with 75% ethanol reflux extraction was concentrated to give the extract, extract system solvent separation method were isolated , and they were the antibacterial activity test . Macroreticular resin method, was separated and purified by column chromatography on silica gel , dextran gel column chromatography and recrystallized from petroleum ether, chloroform , ethyl acetate and n-butanol portion . Conclusion : wood stems from the pig stomach , 25 compounds were isolated , and based on the physical and chemical constants and spectral data ( 1H- NMR , 13C - NMR ) and literature control law identified 20 compounds of the structure , structure identification : clove Lignans (1), quercetin ( 2 ) , of Scoparone ( 3 ) , scopolamine lactone ( 4 ) , Fraxidin ( 5 ) , 4 - hydroxy-3 ,5 - dimethoxybenzaldehyde acrolein (6), 4 - hydroxy - 3 - the methoxybenzene acrolein ( 7 ) , Coniferyl Alcohol (8) , the hydroxy - benzoic acid ( 9) , vanillic acid (10) , syringic acid (11) , syringaldehyde (12) , o-phenylenediamine dimethyl Dioxide (13), vanillic acid 4 - O -beta -D - glucopyranoside glycoside ( 14 ) , 3 - ( 1 -C -beta - glucose) -2,6 - dihydroxy-5 - A benzoic acid (15), mannitol ( 16 ) , the β - daucosterol (17), β- sitosterol ( 18) , nonadecanoic acid (19), dibutyl phthalate (20) . The compound (1), ( 2 ) , ( 5) , ( ??7) , ( ??8) , (9) , (12) , (13) , (14) , (15) , (16) , (19) , ( 20) for the first time isolated from fish bone wood genus . Antimicrobial experimental research and the result showed that the compound (1) , ( 3 ) , (4) , (7) , ( ??11) , (14) : the compound (1) having a high antibacterial activity, and (3 ) and (7 ) have moderate antimicrobial activity, (14) having a low antibacterial activity of these compounds on the Aspergillus niger no antibacterial activity .
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