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Turmeric Bcl-2 and Bax and p53 expression after vascular injury in rats serum experimental study

Author: DongChuan
Tutor: ChangYanYan
School: Liaoning University of Traditional Chinese Medicine
Course: Traditional Chinese Medicine
Keywords: Turmeric Bcl-2 Bax p53
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 19
Quote: 0
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Abstract


Objective: To investigate turmeric fried apoptotic factors Bcl-2 and Bax and p53 expression changes. Speculated that the vascular endothelial cell damage and regulation of traditional Chinese medicine turmeric fried possible mechanisms of endothelial cell apoptosis, the \Materials and methods: analog intimal injury surgery cause the rat acute vascular intimal injury, of turmeric Section 36, 36 model group and the normal group, sham group and 36 and 36 served as controls. Fried turmeric group analog intimal injury postoperative the general routine turmeric the fried 100mg/Kg/d gavage; model group analog intimal injury postoperative general conventional saline 3ml / d orally; sham group laparotomy suture insert a balloon catheter; normal control group not using any interventions. And simulate postoperative days 3, 7, 14, using light and electron microscopy rat endothelial cell structure changes; 144 serum Bcl-2 and Bax and p53 expression changes detected by ELISA. Results: 1. Medication for seven days after the light microscope visible turmeric fried injury model group 7 days vascular damage local endothelial loss and intimal damage at the degree of stenosis visible mitigate, but there are still differences and normal control group, lumen area was significantly smaller than that of the control uninjured side. Electron microscope after intimal injury on the third day visible the endothelial cells columnar irregular, cell shrinkage, the cytoplasmic stain nuclear fragmentation. Seen the release of a small number of apoptotic bodies. Significantly increased on the seventh day, the cell necrosis. Model group, the cells showed obvious necrosis, a large number of fat-soluble and necrotic material within the lipid of fried turmeric group cell necrosis and the other control group significantly reduced only see a small amount of apoptosis debris. 3. Bcl-2 and Bax and p53 expression changes 3.1 turmeric 3 days after drug intervention the turmeric the fried group and model group, sham group Bax content reduce Bcl-2 levels increased, P lt; 0 .05; fried group p53 content compared with model group difference P lt; 0 .05, compared with the sham group was not significantly different, P> 0.05. Compared with the normal control group, P> .05. 3.2 in drug intervention to seven days, after 14 days of fried turmeric with the model group, sham group Bax decreased Bcl-2 levels increased; P lt; 05; of fried turmeric group p53 content with the model group, sham group, there were differences in P lt; 0 .05. Compared with the normal control group, P> 0. 05. Conclusion: 1. Changes after light and electron microscopy simulation intimal injury rat endothelial cell structure and a clear protective effect of turmeric on vascular endothelial Herbal medicine turmeric fried intervention can upregulate rat Bcl-2 protein expression of Bax protein and p53 protein expression was significantly decreased, so that ratio of Bax/Bc1-2 increases to inhibit excessive apoptosis of endothelial cells. The ratio of the two is to decide whether the cells undergo apoptosis regulatory factors. Turmeric can block the cell cycle to reduce the expression of the P53 protein rapidly restore endothelial function, maintain a complete vascular endothelial structure. Pathophysiological process. Apoptotic factors Bcl-2 and Bax and p53 involved after PCI restenosis \Detection of serum Bcl-2 and Bax and p53 content anomalies may contribute to the assessment after PCI apoptotic factors on vascular endothelial function.

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