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Halophilic microorganisms long-term growth in the high- salt environment , has a unique gene , cell structure and function , extreme microbial resources is very important to have a wide range of applications in food, medicine , cosmetics , agriculture, environment , has become international research focus. China has a vast , long coastline , the southeast coastal areas dotted with hundreds of saltworks land types of salt lakes, salt mines and high salt environment halophilic microorganisms Screening and identification work is still far from enough. In this thesis , we sampled the Yantian soil in Yancheng , Jiangsu and isolated a Gram-positive , strictly aerobic moderately halophilic bacteria Y32 . By its heterogeneous taxonomic study showed that an optimum NaCl concentration of 6-8% (w / v), and the optimum growth pH range of 7.0-8.0 , and the optimum growth temperature of 35-40 ° C . Oxidase and catalase positive, nitrate reduction capability, may be hydrolyzed of escin and starch not hydrolyzed casein , L-tyrosine , gelatin and urea . Growth process does not produce H2S and indole , MR and VP test was negative . Cells the major fatty acids were anteiso - C15: 0 , iso -C15 : 0 , iso- C16 : 0, iso- C14 : 0, anteiso -C17 : 0 and C16: 1ω7calcohol . Major respiratory quinone MK-7 ( menaquinone -7) types of cell wall peptidoglycan meso-DAP (meso- diaminopimelic acid ) . DNA ( G C ) content was 40.3mol%. A standard strain bacteria with 4 Pontibacillus the 16SrRNA gene homology distribution of 96.0% -97.8 % . The Y32 bacteria 16SrRNA gene homology highest standard strains Pontibacillus marinusBH030004T DNA-DNA hybridization was 41.8 %. Phenotype through the system , the chemical analysis and the systematic taxonomy analysis shows the close relationship of the respective standard strains of Y32 and Pontibacillus case but its unique distinction . The Y32 the strains belong Pontibacillus genus a new species , it is recommended that and named Pontibacillus yanchengensis sp.nov . The strain mode strain Y32T (= CCTCCAB209311T = NRRL B-59408T), the U.S. Northern Agricultural Institute Culture Collection heart ( NRRL ) deposited at China Type Culture Collection (CCTCC) , respectively .
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