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Sulforaphane (l-isothiocyanate -4 - methylsulfonyl butane, Sulforaphane, l-isothiocyanato-4-methysulfiny-butane) is glucose Radish glycosides (glucosinolates, that kind of glucosinolates) The myrosinase enzyme or acid hydrolysis of a class of isothiocyanate salt, found in vegetables is by far the strongest anti-cancer activity of the active substance. Using modern plant tissue culture techniques have direct access to secondary metabolites used in drug production, with the protection of resources, short growth cycle, from regional and seasonal restrictions, individual differences, ease of industrial production and other advantages. This study established a carrot callus induction and training system, investigated the effects of exogenous additives, different carbon sources, different illumination conditions and radish harvest time on the callus growth and formation of sulforaphane effects. The results showed that: 1. Carrot callus induction and subculture best medium was MS 0.1 mg · L -1 2,4-D 2.0 mg · L -1 6-BA and MS 3.0 mg · L -1 6-BA 0.5 mg · L -1 NAA; 2. response surface experiments, most of sulforaphane best extraction conditions were: extraction solvent: dichloromethane, liquid-solid ratio: 22.9, extraction time: 3.68h, extraction temperature: 22.4 ℃, extraction two times; 3 phenylalanine and valine on radish more proliferation of tissue injury significantly promoted. Add both the concentration of 10 mg · L -1 , the multiplication respectively 11.17 and 10.28 times, phenylalanine simultaneously can significantly promote callus sulforaphane content and yield, Methionine on radish callus proliferation and sulforaphane content does not promote the role; 4 silver nitrate carrot callus proliferation inhibition, but the concentration of 10 mg · L -1 , the content of sulforaphane and output reached 152.51μg · g -1 and 41.77μg · flask -1 ; 5.β-cyclodextrin concentration of added 5 mg · L -1 and 20 mg · L -1 , the carrot callus growth is controlled respectively 2.5 and 2.3 times, but sulforaphane showed no catalytic role. Salicylic acid inhibition of callus proliferation radish, but the content of sulforaphane and yield a significant role in promoting; 6 callus proliferation radish best source of sugar is sucrose, the optimum concentration was added 25 g · L < sup> -1 . Maltose at 15 g · L -1 is the highest content of sulforaphane, and sulforaphane highest yield is 35 g · L -1 glucose; 7.LED red radish cells can significantly improve sulforaphane content and yield, LED yellow and green the most unfavorable sulforaphane synthesis; 8 callus proliferation reached the maximum amount of the first 30 d, and its content of sulforaphane 14 d reached the maximum. Production on sulforaphane, section 14d of the best harvest callus.
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