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Proportional Influence of Th 1/Th2 Lymphocytes After Transferring the Reconstruction of PcDNA3.0/ p27kip1 Eukaryotic Expression Plasmid into Asthma Model of Mice

Author: SunYang
Tutor: ChenPing
School: Fourth Military Medical University
Course: Internal Medicine
Keywords: P27 Gene therapy Immune regulation Asthma
CLC: R562.25
Type: Master's thesis
Year: 2010
Downloads: 13
Quote: 0
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Abstract


Aim:We investigated the effects of the proportional imbalance of Th1/Th2 in allergic asthma model by transferring the reconstruction of PcDNA3.0/ p27 kip1 eukaryotic expression plasmid into allergic asthma model. Methods: KM mice were distributed into three groups: healthy control mice(group A); ovalbumin(OVA) sensitized/challenged mice(group B); OVA sensitized/challenged mice intratracheally administered with PcDNA3.0/ p27 kip1 plasmid(plasmid-treated mice, group C). KM mice were sensitized and challenged intratracheally with OVA, then transfected with the reconstructed PcDNA3.0/ p27 kip1 eukaryotic expression plasmid which was constructed by our laboratory crew through vein injection. The P27 protein expression in lung after 36h of transfection was analyzed by western-blot; Flow cytometry was used to analyze the proportion of CD4 (+)and CD8(+) T lymphocyte subsets in the spleen . The serum levels of IL-4、IL-5、IL-13、IFN- γwere measured by ELISA.Results: Compared to the group A, the expression of P27 protein was significantly reduced,the proportion of CD4(+)lymphocytes、serum levels of IL-4、IL-5、IL-13 was significantly higher but CD8(+)lymphocytes and IFN-γwas significantly decreased in group B. However, in group C, the expression of P27 protein was significantly increased; the proportion of CD4 (+) lymphocytes、serum levels of IL-4、IL-5、IL-13 was significantly decreased but CD8 (+) lymphocytes、IFN-γwas significantly increased than those in group B . Conclusion:P27 could be expressed in lung of plasmid-treated mice and regulate the proportion of Th1/Th2 subset by means of adjusting cell cycles, This method may serve as a potential therapeutic option for the treatment of asthma..

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CLC: > Medicine, health > Internal Medicine > Respiratory system and chest diseases > Trachea and bronchial disease > Bronchial disease > Bronchial asthma
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